| Project/Area Number |
24658083
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
DOI Katsumi 九州大学, (連合)農学研究科(研究院), 講師 (40253520)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Kosuke 九州大学, 大学院農学研究院, 准教授 (00192170)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | phage / Thermus / Inoviridae / replication / genome / 好熱性ウイルス / Thermus thermophilus / Thermus aquaticus / Inovirus / DNA複製 / ゲノム / replicative form / 一本鎖DNA / 溶原性 |
|---|
| Research Abstract |
Isolation of thermophilic bacteriophages were carried out with geothermal waters and sediments in hot springs from Obama hot spring and Komatsu-jigoku hot spring. Isolated phages, phi OH3, phi OH16, phi KJ3, phi KJ4 and phi KJ5, were examined with plaque formation, morphology, host range, replication and genome structure. All isolated phages were categorized into Inoviridae, and predicted ORFs on these phages showed high similarities together. These results suggested Thermus filamentous phages are resemble together, regardless of isolating points.
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