Construction of novel molecular mechanism on polysaccharide-forming enzyme and its application
| Project/Area Number |
24658089
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIMURA Atsuo 北海道大学, (連合)農学研究科(研究院), 教授 (90186312)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 多糖 / ドメイン / 合成 |
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| Research Abstract |
Purpose of this research is the elucidation of structural elements of polysaccharide-producing enzyme and middle-size-oligosaccharide-producing enzyme. Furthermore, the formation of new oligosaccharide is also purpose of this study by controlling the structural element. The obtained results were as follows; (1) Polysaccharide-producing enzyme: Catalytic amino acids were two Glu. Polysaccharide-forming structural element was in the C-terminal region of enzyme. (2) Middle-size-oligosaccharide-producing enzyme: Three-dimensional structure was elucidated, and catalytic residues were Asp and Glu. Mutation analyses of two aromatic residues at sites distant from catalytic center revealed that one residue improved the enzyme function.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Structural elucidation of cyclization mechanism ofα-1,6-glucan by Bacillus circulans T3040 cycloisomaltooligo- saccharide glucanotransferase2014
Author(s)
Nobuhiro Suzuki, Zui Fujimoto, Young-Min Kim, Mitsuru Momma, Naomi Kishine, Ryuichiro Suzuki, Shiho Suzuki, Shinsichi Kitamura, Mikihiko Kobayashi, Atsuo Kimura, Kazumi Funane
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Journal Title
J Biol Chem
Volume: 288(26): in press
Issue: 17
Pages: 12040-12051
DOI
Related Report
Peer Reviewed
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[Journal Article] A novel mechanism for promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine2013
Author(s)
Shinoki A, Lang W, Thawornkuno C, Kang HK, Kumagai Y, Okuyama M, Mori H, Kimura A, Ishizuka S, Hara H
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Journal Title
Food Chem
Volume: 136(2)
Issue: 2
Pages: 293-296
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Degree of polymerization in dietaryα-1,6-glucosaccharides modulates symptom of experimental colitis in rats2012
Author(s)
Hitoshi Iwaya, Jae-Sung Lee, Shinya Yamagishi, Aki Shinoki, Weeranuch Lang, Hee- Kwon Kang, Masayuki Okuyama, Haruhide Mori, Hiroshi Hara, Atsuo Kimura, Satoshi Ishizuka
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Journal Title
Plos One
Volume: 7(11)
Issue: 11
Pages: e50658-e50658
DOI
Related Report
Peer Reviewed
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[Presentation] Molecular mechanism of novel enzymes forming megalosaccharides2014
Author(s)
Yuya Kumagai, Juri Sadahiro, Weeranuch Lang, Young-Min Kim, Michiyo Kanegae, Masayuki Okuyama, Haruhide Mori, Kazumi Funane, Zui Fujimoto, Satoshi Ishizuka, Hiroshi Hara, Doman Kim, Atsuo Kimura
Organizer
Korean Society for Microbiology and Biotechnology
Place of Presentation
BEXCO(Busan、韓国)
Related Report
Invited
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