| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Purpose of this research is the elucidation of structural elements of polysaccharide-producing enzyme and middle-size-oligosaccharide-producing enzyme. Furthermore, the formation of new oligosaccharide is also purpose of this study by controlling the structural element. The obtained results were as follows; (1) Polysaccharide-producing enzyme: Catalytic amino acids were two Glu. Polysaccharide-forming structural element was in the C-terminal region of enzyme. (2) Middle-size-oligosaccharide-producing enzyme: Three-dimensional structure was elucidated, and catalytic residues were Asp and Glu. Mutation analyses of two aromatic residues at sites distant from catalytic center revealed that one residue improved the enzyme function.
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