Identification of novel PTMs

• Project/Area Number: 24658091
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Tohoku University (2013); The University of Tokyo (2012)
• Principal Investigator: YOKOYAMA Atsushi 東北大学, 医学(系)研究科(研究院), 助教 (20572332)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 翻訳後修飾 / 細胞応答 / プロテオミクス

Research Abstract

In this project, we succeeded to identify at least one novel PTM. As a substrate of the novel PTM, we utilized histone proteins. To validate the existence of the novel PTM, we performed in vitro modification assays with radio-active compounds. Furthermore, we succeeded to generate the antibodies recognizing the PTM. These results are significant for future experiments for analyzing the novel PTM.

Research Products

• [Journal Article] Is heparan sulfate in the glomerular basement membrane necessary for the etiology of diabetic nephropathy (2014)
  • Author(s): Saito-Hakoda A, Kogure N, Yoshikawa T, Shimizu K, Iki Y, Sato I, Kudo M, Uruno A, Matsuda K, Parvin R, Sugawara K, Yokoyama A, Ito S, Sugawara A
  • Journal Title: J Biomol Res Ther Volume: vol.3 Issue: 02
  • DOI: 10.4172/2167-7956.1000e127
  • Peer Reviewed
• [Journal Article] Angiotensin II receptor blockers differentially affect CYP11B2 expression in human adrenal H295R cells. (2014)
  • Author(s): Matsuda K, Uruno A, Kogure N, Sugawara K, Shimada H, Nezu M, Saito-Ito T, Iki Y, Kudo M, Shimizu K, Sato I, Yoshikawa T, Satoh F, Ito R, Yokoyama A, Rainey WE, Saito-Hakoda A, Ito S, Sugawara A.
  • Journal Title: Mol Cell Endocrinol. Volume: 383(1-2) Issue: 1-2 Pages: 60-68
  • DOI: 10.1016/j.mce.2013.12.004
  • Peer Reviewed
• [Journal Article] ARB affects nicotine-induced gene expression profile in human coronary artery endothelial cells. (2014)
  • Author(s): Kudo M, Matsuda K, Sugawara K, Iki Y, Kogure N, Saito-Ito T, Shimizu K, Sato I, Yoshikawa T, Uruno A, Yokoyama A, Saito-Hakoda A, Ito S, Sugawara A.
  • Journal Title: World Journal of Hypertension. Volume: 4(1) Issue: 1 Pages: 7-14
  • DOI: 10.5494/wjh.v4.i1.7
  • Peer Reviewed
• [Journal Article] TET3-OGT interaction increases the stability and the presence of OGT in chromatin. (2014)
  • Author(s): 伊藤亮、桂章吾、島田洋樹、土屋光、羽田政司、奥村知子、菅原明、横山敦
  • Journal Title: Genes to Cells Volume: 19 Issue: 1 Pages: 52-65
  • DOI: 10.1111/gtc.12107
  • Peer Reviewed
• [Journal Article] The androgen receptor in health and disease (2013)
  • Author(s): Matsumoto T, Sakari M, Okada M, Yokoyama A, Takahashi S, Kouzmenko A, Kato S
  • Journal Title: Annu Rev Physiol Volume: vol. 75 Issue: 1 Pages: 201-224
  • DOI: 10.1146/annurev-physiol-030212-183656
  • Peer Reviewed
• [Journal Article] JMJD5, a Jumonji C(JmjC) domain-containing protein, negatively regulates osteoclastogenesis by facilitating NFATc1 protein degradation (2012)
  • Author(s): Youn, M.-Y., Imai, Y., et al.
  • Journal Title: J Biol Chem Volume: 287 Issue: 16 Pages: 12994-13004
  • DOI: 10.1074/jbc.m111.323105
  • Peer Reviewed
• [Journal Article] Identification of Post-translational Modifications in Peroxisome Proliferator-activated Receptor gamma Using Mass Spectrometry
  • Author(s): Katsura S, Okumura T, Ito R, Sugawara A, Yokoyama A
  • Journal Title: PPAR Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] Identification of myelin transcription factor 1 (Myt1) as a Subunit of the Neural Cell Type-specific Lysine-specific demethylase 1 (LSD1) Complex
  • Author(s): Yokoyama A, Igarashi K, Sato T, Takagi K, Otsuka I. M, Shishido Y, Baba T, Ito I, Kanno J, Ohkawa Y, Morohashi K, Sugawara A
  • Journal Title: J Biol Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Retinoic acid receptor-αup-regulates proopiomelanocortin gene expression in AtT20 corticotroph cells
  • Author(s): Uruno A, Saito-Hakoda A, Yokoyama A, Kogure N, Matsuda K, Parvin R, Shimizu K, Sato I, Kudo M, Yoshikawa T, Kagechika H, Iwasaki Y, Ito S, Sugawara A
  • Journal Title: Endocr J Volume: (in press)
  • NAID: 130004677954
  • Peer Reviewed
• [Presentation] 組織特異的なヒストン修飾因子複合体の解析 (2013)
  • Author(s): 横山敦
  • Organizer: 第1回疾患エピゲノムコアセンターミニシンポジウム～未知の生命現象・疾患の解明を目指して～
  • Place of Presentation: 宮城
  • Year and Date: 2013-10-26
• [Presentation] 遺伝子発現を制御する新たな翻訳後修飾の探索 (2013)
  • Author(s): 横山敦
  • Organizer: 第16回がん・エピゲノム研究会
  • Place of Presentation: 宮城
  • Year and Date: 2013-07-17
• [Presentation] 遺伝子発現を制御する新たな翻訳後修飾の探索 (2012)
  • Author(s): 横山敦
  • Organizer: 新学術領域研究「性差構築の分子基盤」領域会議
  • Place of Presentation: 和歌山
  • Year and Date: 2012-10-15
• [Presentation] 遺伝子発現を制御する新たな翻訳後修飾の探索 (2012)
  • Author(s): 横山敦
  • Organizer: 新学術領域研究「性差構築の分子基盤」領域会議
  • Place of Presentation: 和歌山ﾏﾘｰﾅｼﾃｨﾛｲﾔﾙﾊﾟｲﾝｽﾞﾎﾃﾙ
• [Presentation] 組織特異的なヒストン修飾因子複合体の解析
  • Author(s): 横山敦
  • Organizer: 第１回 疾患エピゲノムコアセンター ミニシンポジウム～未知の生命現象・疾患の解明を目指して～
  • Place of Presentation: 東北大学医学部（宮城県仙台市）
  • Invited
• [Presentation] 遺伝子発現を制御する新たな翻訳後修飾の探索
  • Author(s): 横山敦
  • Organizer: 第１６回がん・エピゲノム研究会
  • Place of Presentation: 艮陵会館 記念ホール（宮城県仙台市）
  • Invited