Elucidation of metal physiological roles of amyloid-like proteins in plants

• Project/Area Number: 24658093
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Shizuoka University
• Principal Investigator: HARA Masakazu 静岡大学, グリーン科学技術研究所, 教授 (10293614)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 重金属耐性 / アミロイド / 植物 / デハイドリン / 天然変性蛋白質

Outline of Final Research Achievements

Amyloids are proteins which are related to the brain and nerve system disorder. The proteins change their structures from disordered states to aggregate forms under the presence of metal ions. The established theory regarding the significance of amyloid formation in organisms has not been accepted. This study showed that plants possess amyloid-like proteins which contribute to the heavy metal tolerance in plants. It is known that the amyloid-like proteins which accumulated in plant seeds allow the seeds to maintain their drought tolerance for long periods of time. These results suggest that the amyloid-like proteins are distributed throughout the organisms including plants and are related to their stress tolerance.

Research Products

• [Journal Article] Dehydrin expression in seeds and maturation drying: a paradigm change (2014)
  • Author(s): Alzahraa Radwan, Masakazu Hara, Maik Kleinwächter, Dirk Selmar
  • Journal Title: Plant Biology Volume: 16 Issue: 5 Pages: 853-855
  • DOI: 10.1111/plb.12228
  • Peer Reviewed / Open Access
• [Journal Article] Efficient purification of cryoprotective dehydrin protein from the radish (Raphanus sativus) taproot (2014)
  • Author(s): Masakazu Hara, Saki Uchida, Takae Murata, Hermann Wätzig
  • Journal Title: European Food Research and Technology Volume: 239 Issue: 2 Pages: 339-345
  • DOI: 10.1007/s00217-014-2228-6
  • NAID: 120005596546
  • Peer Reviewed / Open Access
• [Journal Article] Precise, fast and flexible determination of protein interactions by Affinity Capillary Electrophoresis: Part 2: Cations (2013)
  • Author(s): Sabine Redweik, Claudia Cianciulli, Masakazu Hara, Yuanhong Xu, Hermann Wätzig
  • Journal Title: Electrophoresis Volume: 34 Issue: 12 Pages: 1812-1819
  • DOI: 10.1002/elps.201300050
  • Peer Reviewed
• [Journal Article] A KS-type dehydrin and its related domains reduce Cu-promoted radical generation and the His residues contribute to the radical-reducing activities (2013)
  • Author(s): Masakazu Hara, Mitsuru Kondo, Takanari Kato
  • Journal Title: Journal of Experimental Botany Volume: 64 Issue: 6 Pages: 1615-1624
  • DOI: 10.1093/jxb/ert016
  • Peer Reviewed
• [Presentation] ヘムと結合したデハイドリンの酵素活性発現に関する研究 (2014)
  • Author(s): 天野翔乃、原 正和
  • Organizer: 日本農芸化学会中部支部第171回例会
  • Place of Presentation: 名古屋大学（愛知県）
  • Year and Date: 2014-10-11
• [Presentation] ヘム存在下におけるデハイドリンの酵素活性発現に関する研究 (2014)
  • Author(s): 天野 翔乃、原 正和
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学（神奈川県）
• [Presentation] 植物の天然変性蛋白質デハイドリンの活性酸素発生抑制活性を規定する配列特性 (2013)
  • Author(s): 原 正和、加藤雄成
  • Organizer: 第31回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 北海道大学（北海道）
• [Presentation] シロイヌナズナのHis-richデハイドリンAtHIRD11の機能研究
  • Author(s): 加藤雄成、篠田友里、原正和、近藤満
  • Organizer: 日本農芸化学会中部支部 第165回例会
  • Place of Presentation: 名古屋大学（愛知県）
• [Presentation] Hisリッチデハイドリンのアデノシン関連物質との結合に関する研究
  • Author(s): 田中 莉子、加藤 雄成、原 正和
  • Organizer: 日本農芸化学会中部支部 第165回例会
  • Place of Presentation: 名古屋大学（愛知県）
• [Presentation] シロイヌナズナデハイドリンによる酵素活性の変化
  • Author(s): 門奈修平、加藤雄成、原正和
  • Organizer: 日本農芸化学会中部支部 第165回例会
  • Place of Presentation: 名古屋大学（愛知県）
• [Remarks] 静岡大学農学部 原研究室
  • URL: http://www.agr.shizuoka.ac.jp/abc/envplant/index.html
• [Remarks] 静岡大学農学研究科 植物機能生理学研究室（原）
  • URL: http://www.agr.shizuoka.ac.jp/abc/envplant/index.html
• [Remarks] 植物機能生理学研究室（原）
  • URL: http://www.agr.shizuoka.ac.jp/abc/envplant/index.html