Studies of factors that inhibit the macrophage migration

• Project/Area Number: 24658094
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: KIOKA Noriyuki 京都大学, (連合)農学研究科(研究院), 准教授 (90234179)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: メタボリック症候群 / 糖尿病 / マクロファージ / 細胞遊走 / 細胞接着 / 炎症 / 生活習慣病

Research Abstract

Macrophage invasion into adipose tissues plays a critical role for metabolic syndrome. An adaptor protein, CAP, is involved in the invasion of macrophages. Here we established the assay system for CAP-dependent macrophage migration. First we determined the optimum condition for examining macrophage migration. Then, expression of CAP was depleted in macrophage-like cells by shRNA for CAP. We found that CAP depletion promoted the macrophage migration estimated by xCELLigence. Re-expression of CAP isolated from macrophages rescued the migration. This system can be used for investigation of CAP-dependent macrophage migration.

Research Products

• [Journal Article] Loss of Dlg5 expression promotes the migration and invasion of prostate cancer cells via Girdin phosphorylation (2014)
  • Author(s): Tomiyama, L., Sezaki, T., Matsuo, M., Ueda, K. and Kioka, N.
  • Journal Title: Oncogene Volume: 33 Issue: 9 Pages: 1141-1149
  • DOI: 10.1038/onc.2014.31
  • Peer Reviewed
• [Journal Article] Interaction of the vinculin proline-rich linker region with vinexin α in sensing extracellular matrix stiffness (2014)
  • Author(s): Hiroshi Yamashita, Takafumi Ichikawa, Daisuke Matsuyama, Yasuhisa Kimura, Kazumitsu Ueda, Susan W. Craig, Ichiro Harada, and Noriyuki Kioka
  • Journal Title: Journal of Cell Science Volume: 127 Pages: 1875-86
  • DOI: 10.1242/jcs.133645
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dlg5 interacts with the TGF-beta receptor and promotes its degradation (2013)
  • Author(s): Takuhito Sezaki, Lucia Tomiyama, Yasuhisa
  • Journal Title: FEBS Lett Volume: (in press) Issue: 11 Pages: 1624-1629
  • DOI: 10.1016/j.febslet.2013.04.015
  • Peer Reviewed
• [Journal Article] Vinexin-beta protects against cardiac hypertrophy by blocking the Akt-dependent signalling pathway (2013)
  • Author(s): Chen, K. Gao, L. Liu, Y. Zhang, Y. Jiang, D. S. Wei, X. Zhu, X. H. Zhang, R. Chen, Y. Yang, Q. Kioka. N. Zhang. X
  • Journal Title: Basic research in cardiology Volume: 108 Issue: 2 Pages: 338-338
  • DOI: 10.1007/s00395-013-0338-0
  • Peer Reviewed
• [Journal Article] Dlg5 interacts with the TGF-beta receptor and promotes its degradation (2013)
  • Author(s): Takuhito Sezaki, Lucia Tomiyama, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka
  • Journal Title: FEBS Lett Volume: in press
  • Peer Reviewed
• [Journal Article] Non-specific protein modifications by a phytochemical induce heat shock response for self-defense (2013)
  • Author(s): Kohta Ohnishi
  • Journal Title: PLoS One Volume: 8(3) Issue: 3 Pages: 1-15
  • DOI: 10.1371/journal.pone.0058641
  • Peer Reviewed / Open Access
• [Journal Article] Role of Dlg5/1p-dlg, a membrane-associated guanylate kinase family protein, in epithelial-mesenchymal transition in LLc-PK1 renal epithelial cells (2012)
  • Author(s): Takuhito Sezaki, Kohki Inada, Takayuki Sogabe, Kumiyo Kakuda, Lucia Tomiyama, Yohsuke Matsuno, Takafumi Ichikawa, Michinori Matsuo, Kazumitsu Ueda, Noriyuki Kioka
  • Journal Title: PLoS ONE Volume: 7 Issue: 4 Pages: e35519-e35519
  • DOI: 10.1371/journal.pone.0035519
  • Peer Reviewed
• [Journal Article] ATP hydrolysis-dependent conformational changes in the extracellular domain of ABCA1 are associated with apoA-I binding. (2012)
  • Author(s): Nagao, K., Takahashi, K., Azuma, Y., Takada, M., Kimura, Y., Matsuo, M., Kioka, N. and Ueda, K.
  • Journal Title: J Lipid Res Volume: 53 Issue: 1 Pages: 126-136
  • DOI: 10.1194/jlr.m019976
  • Peer Reviewed
• [Presentation] 結合能と接着斑増強効果を指標としたビネキシンファミリー(Sorbs)タンパク質の機能の比較解析 (2014)
  • Author(s): 喜多真弘、市川尚文、瀬崎拓人、松尾道憲、植田和光、木岡紀幸
  • Organizer: 日本農芸化学会2014
  • Year and Date: 2014-03-29
• [Presentation] 脂肪細胞分化の「細胞外マトリクスの硬さ」依存性に接着斑タンパク質ビンキュリンが必要である (2014)
  • Author(s): 黒田美都、和田洋樹、植田和光、木岡紀幸
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京
• [Presentation] 細胞外マトリクスの硬さの感知におけるビンキュリン-ビネキシンα相互作用 (2013)
  • Author(s): 市川尚文,山下寛,木村泰久,植田和光,木岡紀幸
  • Organizer: 第480回農芸化学会関西支部講演会
  • Place of Presentation: 大阪
  • Year and Date: 2013-07-06
• [Presentation] PIP2- dependent distribution of vinculin to lipid rafts stabilizes vinculin at focal adhesions (2013)
  • Author(s): Ayaka Nagasato, Hiroshi Yamashita, Michinori Matsuo, Kazumitsu Ueda, Noriyuki Kioka
  • Organizer: The 14th International Membrane Research Forum
  • Place of Presentation: 京都
• [Presentation] 接着斑タンパク質ビンキュリンの脂質ラフト局在化にはPIP2との相互作用が必要である (2013)
  • Author(s): 長里彩花、山下寛、市川尚文、松尾道憲、植田和光、木岡紀幸
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
• [Presentation] 細胞外マトリックスの硬さを感知するメカノセンサーとしての"接着斑" (2013)
  • Author(s): 木岡紀幸
  • Organizer: 理研シンポジウム「最先端光計測とライフサイエンスの近未来-バイオラマン2017
  • Place of Presentation: 松山
• [Presentation] PIP2- and vinexin-dependent distribution of vinculin to lipid rafts stabilizes vinculin at focal adhesions. (2013)
  • Author(s): Ayaka Nagasato, Hiroshi Yamashita, Michinori Matsuo, Kazumitsu Ueda and Noriyuki Kioka
  • Organizer: 第６５回日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 細胞外マトリックスの硬さを感知するメカノセンサーとしての“接着斑” (2013)
  • Author(s): 木岡紀幸
  • Organizer: 理研シンポジウム「最先端光計測とライフサイエンスの近未来-バイオラマン2017-
  • Place of Presentation: 松山
  • Invited
• [Presentation] 細胞外マトリクスの硬さの感知におけるビンキュリン-ビネキシンα相互作用 (2013)
  • Author(s): 市川尚文, 山下寛, 木村泰久, 植田和光, 木岡紀幸
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] ビネキシンファミリー（Sorbs）タンパク質の機能の比較解析 (2013)
  • Author(s): 喜多真弘、市川尚文、瀬崎拓人、松尾道憲、植田和光、木岡紀幸
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Interaction between vinculin and vinexin a plays a key role in sensing extracellular matrix stiffness (2013)
  • Author(s): Takafumi Ichikawa, Hiroshi Yamashita, Yasuhisa Kimura, Kazumitsu Ueda, Noriyuki Kioka
  • Organizer: The 14th International Membrane Research Forum
  • Place of Presentation: 京都
• [Presentation] Interaction between vinculin and vinexin is involved in sensing ECM rigidity (2012)
  • Author(s): Noriyuki Kioka, Hiroshi Yamashita, Takafumi Ichikawa, Kazumitsu Ueda
  • Organizer: Signaling by Adhesion Receptors Gordon Conferences
  • Place of Presentation: Waterville, ME, USA
• [Presentation] Vinexin-dependent distrr Cell Biology (2012)
  • Author(s): Hiroshi Yamashita, Ayaka Nagasato, Takafumi Ichikawa, Michinori Matsuo, Kazumitsu Ueda and Noriyuki Kioka
  • Place of Presentation: San Francisco,CA, USA
• [Presentation] 細胞外マトリックスの堅さの感知におけるビンキュリン-ビネキシン相互作用 (2012)
  • Author(s): 木岡紀幸
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡ワークショップ
• [Presentation] 細胞外マトリックスの堅さの感知におけるビンキュリン-ビネキシン相互作用 (2012)
  • Author(s): 木岡紀幸
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Roles of Dlg5, a Crohn’s disease associated protein, as a regulator of TGF-b signals and epithelial to mesenchymal transition (2012)
  • Author(s): Takuhito Sezaki、Kohki Inada、Lucia Tomiyama、Kazumitsu Ueda、Noriyuki Kioka
  • Organizer: 2012 Annual Meeting of The American Society for Cell Biology
  • Place of Presentation: San Francisco,CA, USA
• [Remarks] 京都大学農学研究科応用生命科学専攻細胞生化学分野
  • URL: http://www.biochemistry.kais.kyoto-u.ac.jp/
• [Remarks] 細胞生化学分野研究業績
  • URL: http://www.biochemistry.kais.kyoto-u.ac.jp/gyouseki/gyousekiin.html
• [Remarks] 細胞生化学研究室
  • URL: http://www.biochemistry.kais.kyoto-u.ac.jp/