• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction of high lipid content rice by the molecular mechanism analysis of the lipid synthesis in rice seed

Research Project

Project/Area Number 24658097
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied biochemistry
Research InstitutionKyoto Prefectural University

Principal Investigator

TAKEHIRO Masumura  京都府立大学, 生命環境科学研究科(系), 講師 (50254321)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords植物 / 遺伝子発現 / 脂質 / イネ種子 / アリューロン層 / 転写因子 / 脂質合成遺伝子 / 形質転換イネ
Outline of Final Research Achievements

This study was intended to strengthen the aleurone layer which accumulated functional materials in rice seeds. High-coverage profiling analysis of gene expression in the aleurone layer was performed, and searched for important transcription factors or enzyme genes from the profiling data. The candidate clone was introduced into rice genome to create high content of lipid accumulation in transgenic rice seed.
Under ubiquitin promoter control, the transgenic rice which overexpressed the transcription factor (NFYB) gene which specifically expressed during early differentiation stage of endosperm tissue was created. The seed morphology of the transgenic rice was observed, and lipid content was measured. The starchy endosperm tissues of transgenic seed were reduced, and the lipid contents per seed were increased. We found possibility to improve the lipid synthesis ability by decreasing the site of storage protein synthesis in transgenic seed by this study.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (24 results)

All 2015 2014 2013 2012

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (14 results) (of which Invited: 1 results)

  • [Journal Article] Identification of the region of rice 13 kDa prolamin essential for the formation of ER-derived protein bodies using a heterologous expression system.2015

    • Author(s)
      Takehiro Masumura**, Takanari Shigemitsu**, Shigeto Morita, Shigeru Satoh
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 79 Issue: 4 Pages: 566-573

    • DOI

      10.1080/09168451.2014.991684

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic protein expressions of phosphoenolpyruvate carboxylase in developing rice seeds.2014

    • Author(s)
      Naoki Yamamoto, Masayuki Shimada, Toshio Sugimoto, Yoshikiyo Oji, Takehiro Masumura
    • Journal Title

      Journal of Cereal Science

      Volume: 60 Issue: 2 Pages: 457-480

    • DOI

      10.1016/j.jcs.2014.06.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Concomitant increases of the developing seed phosphoenolpyruvate carboxylase activity and the seed protein content of field-grown wheat with nitrogen supply.2014

    • Author(s)
      Naoki Yamamoto, Toshio Sugimoto, Takehiro Masumura
    • Journal Title

      Agricultural Sciences

      Volume: 5 Issue: 14 Pages: 1558-1565

    • DOI

      10.4236/as.2014.514167

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RNAi-mediated suppression of endogenous storage proteins leadsto a change in localization of overexpressed cholera toxinB-subunit and the allergen protein RAG2 in rice seeds.2014

    • Author(s)
      Kurokawa, S., et al.
    • Journal Title

      Plant Cell Rep.

      Volume: 33 Issue: 1 Pages: 75-87

    • DOI

      10.1007/s00299-013-1513-3

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning, gene expression and functional expression of a phosphoenolpyruvate carboxylase Osppc1 in developing rice seeds: implication of involvement in nitrogen accumulation.2014

    • Author(s)
      Naoki Yamamoto, Tatsuya Kubota, Takehiro Masumura, Naomasa Shiraishi, Kunisuke Tanaka, Toshio Sugimoto, Yoshikiyo Oji
    • Journal Title

      Seed Science Research

      Volume: 24 Issue: 1 Pages: 23-36

    • DOI

      10.1017/s0960258513000354

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] MucoRice-cholera toxin B-subunit, a rice-based oral cholera vaccine, down-regulates the expression of α-amylase/trypsin inhibitor-like protein family as major rice allergens.2013

    • Author(s)
      Kurokawa, S., et al.
    • Journal Title

      J. Proteome Res.

      Volume: 12 Issue: 7 Pages: 3372-3382

    • DOI

      10.1021/pr4002146

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Induction of toxin-specific neutralizing immunity by molecularly uniform rice-based oral cholera toxin B subunit vaccine without plant-associated sugar modification.2013

    • Author(s)
      Yuki, Y., et al.
    • Journal Title

      Plant Biotechnol. J.

      Volume: 11 Issue: 7 Pages: 799-808

    • DOI

      10.1111/pbi.12071

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Accumulation of rice prolamin-GFP fusion proteins induces ER-derived protein bodies in transgenic rice calli.2013

    • Author(s)
      Takanari Shigemitsu, Takehiro Masumura, Shigeto Morita, Shigeru Satoh
    • Journal Title

      Plant Cell Reports

      Volume: 32 Pages: 389-399

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cloning and expression of genes for biosynthesis of very-long-chain fatty acids, the precursors for cuticular wax formation, in carnation (Dianthus caryophyllus L.) petals.2013

    • Author(s)
      Masaya Kawarada, Yoshihiro Nomura, Taro Harada, Shigeto Morita, Takehiro Masumura, Hiroyasu Yamaguchi, Koji Tanase, Masafumi Yagi, Takashi Onozaki, Shigeru Satoh
    • Journal Title

      Journal of the Japanese Society for Horticultural Science

      Volume: 82 Pages: 161-169

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 少量試料による米粉生地の膨化測定2012

    • Author(s)
      奥西智哉、宮下香苗、大江翔太郎、萬代悠太、増村威宏、黒田昌治
    • Journal Title

      日本食品科学工学会誌

      Volume: 59 Pages: 473-475

    • NAID

      10031055001

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] A potential use of phosphoenolpyruvate carboxylase in developing rice seeds.2014

    • Author(s)
      Naoki Yamamoto, Toshio Sugimoto, Ai Sasou, Tatsuya Kubota, Masahiro Shimada, Yuki Saito, Naomasa Shiraishi, Shigeto Morita, Shigeru Sato, Kentaro Yano, Kunisuke Tanaka, Yoshikiyo Oji, Takehiro Masumura
    • Organizer
      4th International Rice Congress
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2014-10-27 – 2014-11-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] ダイズ種子ホスホエノールピルビン酸カルボキシラーゼの情報科学的解析2014

    • Author(s)
      山本直樹、高野知之、矢野健太郎、杉本敏男、増村威宏
    • Organizer
      日本農芸化学会関西支部大会
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Year and Date
      2014-09-19 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] イネ登熟種子中の脂質合成関連遺伝子群の組織別・網羅的発現解析2014

    • Author(s)
      木下由貴、斉藤雄飛、東田潤、土居誠、寺島伸、堺谷荘太、森田重人、佐藤茂、石丸努、近藤始彦、山本直樹、矢野健太郎、増村威宏
    • Organizer
      第32回日本植物細胞分子生物学会
    • Place of Presentation
      岩手
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] コムギ登熟種子ホスホエノールピルビン酸カルボキシラーゼの開花期窒素供給への応答と遺伝子発現2014

    • Author(s)
      山本直樹、高野知之、木下由貴、佐生愛、矢野健太郎、杉本敏男、増村威宏
    • Organizer
      日本農芸化学会第485回関西支部例会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス
    • Year and Date
      2014-07-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 経口コメ型ワクチンMucoRice-CTBにおける主要アレルゲンのプロテオーム解析2013

    • Author(s)
      ○黒河志保、中村里香、目島未央、秦裕子、黒田昌治、竹山夏実、尾山大明、佐藤茂、清野宏、増村威宏、手島玲子、幸義和
    • Organizer
      日本農芸化学会関西支部第479回講演会
    • Place of Presentation
      京都府立大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 日本晴と組換えワクチン発現イネにおけるコメアレルゲンRAG2タンパク質の種子内局在部位2013

    • Author(s)
      ○黒河志保、黒田昌治、目島未央、中村里香、高橋裕子、相良洋、竹山夏実、佐藤茂、清野宏、手島玲子、増村威宏、幸義和
    • Organizer
      本農芸化学会関西支部第480回講演会
    • Place of Presentation
      大阪府立大学
    • Related Report
      2013 Research-status Report
  • [Presentation] イネ種子プロテインボディタイプI(PB-I)に蓄積するプロラミン分子種間の相互作用の解析2013

    • Author(s)
      ◯佐生愛、重光隆成、森田重人、佐藤茂、増村威宏
    • Organizer
      日本農芸化学会関西支部大会
    • Place of Presentation
      広島県立大学
    • Related Report
      2013 Research-status Report
  • [Presentation] イネ種子で発現する貯蔵脂質合成関連遺伝子群の解析2013

    • Author(s)
      ○堺谷荘太、斉藤雄飛、東田潤、土居誠、森田重人、佐藤茂、石丸努、近藤始彦、増村威宏
    • Organizer
      第31回植物細胞分子生物学会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 経口ワクチン用キャリアーを目指したイネ種子PB-Iの特定部位への外来タンパク質局在化と消化酵素耐性に関する研究2013

    • Author(s)
      ○佐生愛、重光隆成、齊藤雄飛、田中愛実、森田重人、佐藤茂、増村威宏
    • Organizer
      第31回植物細胞分子生物学会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 抗菌タンパク質を発現するイネに関する研究2013

    • Author(s)
      ○菊田桃香、佐生愛、重光隆成、森田重人、佐藤茂、増村威宏
    • Organizer
      第31回植物細胞分子生物学会
    • Place of Presentation
      北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 高温障害が米の食味と貯蔵タンパク質へ及ぼす影響2013

    • Author(s)
      増村威宏、重光隆成、後藤双水、齊藤雄飛、石丸努、近藤始彦
    • Organizer
      日本作物学会第235回講演会
    • Place of Presentation
      明治大学生田キャンパス
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] イネ種子における貯蔵脂質合成関連遺伝子群の発現解析2012

    • Author(s)
      堺谷荘太、齊藤雄飛、東田潤、土居誠、森田重人、佐藤茂、石丸努、近藤始彦、増村威宏
    • Organizer
      第30回植物細胞分子生物学会
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Related Report
      2012 Research-status Report
  • [Presentation] PB-I様構造体を形成した形質転換イネカルスの解析2012

    • Author(s)
      重光隆成、森田重人、佐藤茂、増村威宏
    • Organizer
      第30回植物細胞分子生物学会
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 環境ストレスを受けたイネにおける種子貯蔵タンパク質の解析2012

    • Author(s)
      後藤双水、重光隆成、齊藤雄飛、森田重人、佐藤茂、石丸努、近藤始彦、増村威宏
    • Organizer
      第30回植物細胞分子生物学会
    • Place of Presentation
      奈良先端科学技術大学院大学
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi