| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
This study was intended to strengthen the aleurone layer which accumulated functional materials in rice seeds. High-coverage profiling analysis of gene expression in the aleurone layer was performed, and searched for important transcription factors or enzyme genes from the profiling data. The candidate clone was introduced into rice genome to create high content of lipid accumulation in transgenic rice seed.
Under ubiquitin promoter control, the transgenic rice which overexpressed the transcription factor (NFYB) gene which specifically expressed during early differentiation stage of endosperm tissue was created. The seed morphology of the transgenic rice was observed, and lipid content was measured. The starchy endosperm tissues of transgenic seed were reduced, and the lipid contents per seed were increased. We found possibility to improve the lipid synthesis ability by decreasing the site of storage protein synthesis in transgenic seed by this study.
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