Construction of high lipid content rice by the molecular mechanism analysis of the lipid synthesis in rice seed
| Project/Area Number |
24658097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
TAKEHIRO Masumura 京都府立大学, 生命環境科学研究科(系), 講師 (50254321)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 植物 / 遺伝子発現 / 脂質 / イネ種子 / アリューロン層 / 転写因子 / 脂質合成遺伝子 / 形質転換イネ |
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| Outline of Final Research Achievements |
This study was intended to strengthen the aleurone layer which accumulated functional materials in rice seeds. High-coverage profiling analysis of gene expression in the aleurone layer was performed, and searched for important transcription factors or enzyme genes from the profiling data. The candidate clone was introduced into rice genome to create high content of lipid accumulation in transgenic rice seed.
Under ubiquitin promoter control, the transgenic rice which overexpressed the transcription factor (NFYB) gene which specifically expressed during early differentiation stage of endosperm tissue was created. The seed morphology of the transgenic rice was observed, and lipid content was measured. The starchy endosperm tissues of transgenic seed were reduced, and the lipid contents per seed were increased. We found possibility to improve the lipid synthesis ability by decreasing the site of storage protein synthesis in transgenic seed by this study.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Cloning and expression of genes for biosynthesis of very-long-chain fatty acids, the precursors for cuticular wax formation, in carnation (Dianthus caryophyllus L.) petals.2013
Author(s)
Masaya Kawarada, Yoshihiro Nomura, Taro Harada, Shigeto Morita, Takehiro Masumura, Hiroyasu Yamaguchi, Koji Tanase, Masafumi Yagi, Takashi Onozaki, Shigeru Satoh
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Journal Title
Journal of the Japanese Society for Horticultural Science
Volume: 82
Pages: 161-169
Related Report
Peer Reviewed
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[Presentation] A potential use of phosphoenolpyruvate carboxylase in developing rice seeds.2014
Author(s)
Naoki Yamamoto, Toshio Sugimoto, Ai Sasou, Tatsuya Kubota, Masahiro Shimada, Yuki Saito, Naomasa Shiraishi, Shigeto Morita, Shigeru Sato, Kentaro Yano, Kunisuke Tanaka, Yoshikiyo Oji, Takehiro Masumura
Organizer
4th International Rice Congress
Place of Presentation
Bangkok, Thailand
Year and Date
2014-10-27 – 2014-11-01
Related Report
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