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Remediation of environmetal pollution by the recombinant Escherichia coli accumulating cesium in the cell

Research Project

Project/Area Number 24658098
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionKinki University

Principal Investigator

YOSHIOKA Sachiko  近畿大学, 農学部, 助手 (80200939)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kaneyoshi  法政大学, 生命科学部, 准教授 (40351580)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords遺伝子工学 / 環境応答 / 大腸菌 / 遺伝子組換え / セシウム / ゲノム発現 / セシウム輸送タンパク質 / セシウム結合タンパク質 / セシウム除染
Research Abstract

The purpose of this study is to design and construct the recombinant Escherichia coli, accumulating cesium ion in the cell. We showed genomic changes of metal-transporter and metal-binding protein increase the level of intracellular metal in E. coli in the cases of molybdenum and manganese. To identify the genes for cesium-transporter and cesium-binding protein in E. coli, we carried out transcriptome analysis. The result showed three genes, each encoding a putative metal-binding transcription factor, were increased by addition of cesium chloride. The deletion of these genes each increased the sensibility to high concentration of cesium. These results suggest that the identified genes involved in response to extracellular cesium in E. coli and are targets for genomic change to accumulate intracellular cesium ion in a cell.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (21 results)

All 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 7 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] 細胞内でレアメタルを高蓄積する大腸菌のゲノム育種と応用2014

    • Author(s)
      山本兼由
    • Journal Title

      ケミカルエンジニヤリング

      Volume: VOL.59 No.4

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Screening of promoter-specific transcription factors : multiple regulators for the sdiA gene involved in cell division control and quorum sensing2013

    • Author(s)
      Shimada K, Ogasawara H, Yamada K, Shimura M, Kori A, Shimada T, Yamanaka Y, Yamamoto K, Ishihama A
    • Journal Title

      Microbiology

      Volume: 159(Pt 12) Pages: 2501-2512

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the set of genes, including non-annotated morA, under the direct control of ModE in Escherichia coli2013

    • Author(s)
      Kurata, T., Katayama, A., Hiramatsu, M., Kiguchi, Y., Takeuchi, M., Watanabe, T., Ogasawara, H., Ishihama, A., and Yamamoto, K.
    • Journal Title

      J. Bacteriol

      Volume: 195(19) Pages: 4496-44505

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of EnvZ-OmpR two-component system in virulence control of Escherichia coli in Drosophila melanogaster2013

    • Author(s)
      Pukklay, P., Nakanishi, Y., Nitta, M., Yamamoto, K., Ishihama, A., and Shiratsuchi A.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 438(2) Pages: 306-311

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Screening of promoter-specific transcription factors:multiple regulators for the sdiA gene involved in cell division control and quorum sensing.2013

    • Author(s)
      Shimada K, Ogasawara H, Yamada K,Shimura M, Kori A, Shimada T, Yamanaka Y, Yamamoto K, Ishihama A
    • Journal Title

      Microbiology

      Volume: 159 Pages: 2501-2512

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the set of genes, including non-annotated morA, under the direct control of ModE in Escherichia coli2013

    • Author(s)
      Kurata T, Katayama A, Hiramatsu M, Kiguchi Y, Takeuchi M, Watanabe T, Ogasawara H, Ishihama A Yamamoto K
    • Journal Title

      J. Bacteriol

      Volume: 195 Pages: 4496-44505

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inovolvement of EnvZ-OmpR two-component system in virulence control of Escherichia coli in Drosophila melanogaster2013

    • Author(s)
      Pukklay P, Nakanishi Y, Nitta M, Yamamoto K, Ishihama A Shiratsuchi A
    • Journal Title

      Biochem. Biopys. Res. Commun

      Volume: 438 Pages: 306-311

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli2012

    • Author(s)
      Shimada, T., Katayama, Y., Kawakita, S., Ogasawara, H., Nakano, M., Yamamoto, K., Ishihama, A.
    • Journal Title

      Microbiologyopen

      Volume: 1 Issue: 4 Pages: 381-394

    • DOI

      10.1002/mbo3.42

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel regulation targets of the metal-response BasS-BasR two-component system of Escherichia coli2012

    • Author(s)
      Ogasawara, H., Shinohara, S., Yamamoto, K., and Ishihama, A.
    • Journal Title

      Microbiology

      Volume: 158(Pt 6) Pages: 1482-1492

    • Related Report
      2013 Final Research Report
  • [Journal Article] Induction of YdeO, a regulator for acid resistance genes, by ultraviolet irradiation in Escherichia coli2012

    • Author(s)
      Yamanaka, Y., Ishihama, A., and Yamamoto, K.
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 76(6) Pages: 1236-1238

    • NAID

      10030818581

    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸菌GadEによるゲノム発現制御メカニズムの解明2014

    • Author(s)
      山中幸、志波優、山本健太郎、川岸郁朗、吉川博文、石浜明、山本兼由
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸菌システイン合成遺伝子群を制御するCysB-Cblカスケード2014

    • Author(s)
      山本兼由、山田佳代子、岩田紀子、小澤貴博、富山あや乃、千野拓馬、石井絵里、鈴木孝太、尾崎友紀、石浜明
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2013 Final Research Report
  • [Presentation] システイン代謝遺伝子転写因子YdcNの包括転写因子としての機能2014

    • Author(s)
      中野雅博、多田麻里永、石浜明、山本兼由
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸菌GadEによるゲノム発現制御メカニズムの解明2014

    • Author(s)
      山中 幸、志波 優、山本健太郎、川岸郁朗、吉川博文、石浜 明、山本兼由
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌システイン合成遺伝子群を制御するCysB-Cblカスケード2014

    • Author(s)
      山本兼由、山田佳代子、岩田紀子、小澤貴博、富山あや乃、千野拓馬、石井絵里、鈴木孝太、尾崎友紀、石浜 明
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] システイン代謝遺伝子転写因子YdcNの包括転写因子としての機能2014

    • Author(s)
      中野雅博、多田麻里永、石浜 明、山本兼由
    • Organizer
      日本農芸化学会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌核様体形成の主要蛋白H-NSのシステマティックな制御機能解析2013

    • Author(s)
      山本兼由、山中幸、大島拓、山田佳代子、岩田紀子、大村悦子、崎井裕貴、中川日出子、渡會祥、曽和義幸、川岸郁朗、石浜明
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸菌の二成分制御系レスポンスレギュレーター間における転写制御ネットワーク2013

    • Author(s)
      吉多美祐、安達友美、渡邊宏樹、石浜明、山本兼由
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫)
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸菌核様体形成の主要蛋白H-NSのシステマティックな制御機能解析2013

    • Author(s)
      山本兼由、山中 幸、大島 拓、山田佳代子、岩田紀子、大村悦子、崎井裕貴、中川日出子、渡會 祥、曽和義幸、川岸郁朗、石浜 明
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大腸菌の二成分制御系レスポンスレギュレーター間における転写制御ネットワーク2013

    • Author(s)
      吉多美祐、安達友美、渡邊宏樹、石浜 明、山本兼由
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2013 Annual Research Report
  • [Remarks] 新聞記事 日刊工業新聞 (2014年1月15日) 「法政大、大腸菌でレアメタルを高濃度に蓄積することに成功」

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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