| Project/Area Number |
24658102
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
MATSUURA Hideyuki 北海道大学, (連合)農学研究科(研究院), 准教授 (20344492)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | Jasmonic acid / 植物ホルモン / 植物の傷害応答 / ジャスモン酸 / ジャスモン酸アミノ縮合体 / 配糖体化ジャスモン酸 |
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| Outline of Final Research Achievements |
In order to uncover controling system of jasmonate signaling pathway, we attempted to find out UDP-glucose independent transglucosylation enzyme, and OsBGlu1 was picked up to afford 12-glucopyranosyl jasmonic acid and 12-glucopuranosyljasmonoyl L-isoleucine. The biological activities of jasmonic acid, 12-hydroxyjasmonoyl L-isoleucine and 12-glucopyranosyljasmonoyl L-isoleucine were comparied, which revealed that jasmonic acid has most effective activity and 12-hydroxyjasmonoyl L-isoleucine has the lowest activity. Thus, our project showed that transglucosylation reaction mediated by OsBGlu1 is important pathway to control the activity of jasmonic acid.
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