| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
In the LC-MSMS spectral analysis, assigning C-terminus y- and N-terminus b-ions are important to sequence the peptides. However, these have been based on empirical. In this study, the reporter established a method to theoretically distinguish N-terminus b-ions and C-terminus y-ions by introducing mass difference in at N- or C-terminus with derivatizations. The reporter also developed software named "MSMS Search Tool" which automatically selects y-ions and b-ions using raw mass data (ASCII format).
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