Development of effective method selectively detecting y and b ions in the LC-MS/MS
| Project/Area Number |
24658104
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | LC-MS/MS / 配列決定 / イオン帰属 / アイソトポログ / 自動化 / MSMS解析 / ペプチド配列 / イオン識別 / ESIMS / CID-MS/MS / アイソトポマー / イオン区別 |
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| Research Abstract |
In the LC-MSMS spectral analysis, assigning C-terminus y- and N-terminus b-ions are important to sequence the peptides. However, these have been based on empirical. In this study, the reporter established a method to theoretically distinguish N-terminus b-ions and C-terminus y-ions by introducing mass difference in at N- or C-terminus with derivatizations. The reporter also developed software named "MSMS Search Tool" which automatically selects y-ions and b-ions using raw mass data (ASCII format).
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Report
(3 results)
Research Products
(67 results)
