| Project/Area Number |
24658124
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Food science
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| Research Institution | Kyushu University |
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Principal Investigator |
FUJIMURA Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
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| Co-Investigator(Kenkyū-buntansha) |
SETOYAMA Daiki 九州大学, 先端融合医療レドックスナビ研究拠点, 特任助教 (30550850)
MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | メタボロミクス / MALDI-MS / マトリックス / 質量分析イメージング / 代謝物プロファイリング / 機能性食品 / 質量分析 / 緑茶カテキン |
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| Research Abstract |
In this study, we attempted to develop a technique and methodology, understanding of MALDI ionization rationale and synthesis of new matrix, for improvement of molecular coverage and detection sensitivity toward high-resolution metabolite analysis on the basis of MALDI-MS capable of sensitively detecting metabolites from trace amount of cells. We succeeded in newly proposing a methodology of chemometric prediction of MALDI ionization based on the quantitative structure-property relationship (QSPR) analysis and in newly generating highly sensitive matrix for metabolites. In addition, we found new molecular mechanism, mitochondrial regulation system, of oxidative stress, which is often observed in biological responses against food factors.
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