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Investigation of target proteins by isothiocyanate with fluorescent probe

Research Project

Project/Area Number 24658127
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionUniversity of Hyogo

Principal Investigator

KATO Yoji  兵庫県立大学, 環境人間学部, 教授 (30305693)

Co-Investigator(Kenkyū-buntansha) 中村 宜督  岡山大学, 環境生命科学研究科, 准教授 (60324381)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsイソチオシアネート / 蛍光 / アミノ付加体 / 標的タンパク質 / 生理機能
Research Abstract

Though isothiocyanates have some biological functions such as anti-cancer or anti-bacterial activities, the mechanisms has not been shown in detail. We found the covalent adduction of ITC with lysine in a protein at physiological conditions. In this study, we focused in fluorescent character of ITC by using FITC. A monoclonal antibody to fluorescent structure has been prepared and characterized and then applied to fluorescent staining of cells. The multiple ITC adducted proteins were then detected in a gel by fluorescent scanning or by specific antibody. The identification of ITC adducted proteins remains to be done.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2013 Other

All Presentation (2 results) Remarks (2 results)

  • [Presentation] 蛍光色素FITC修飾タンパク質に対する抗体の作製とその応用2013

    • Author(s)
      松本亮,礒見みづき,中村宜督,北元憲利,加藤陽二
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2013-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 蛍光色素FITC修飾タンパク質に対する抗体の作製とその応用2013

    • Author(s)
      松本亮, 礒見みづき, 中村宜督, 北元憲利, 加藤陽二
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東北大学川内北キャンパス(宮城県)
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.shse.u-hyogo.ac.jp/yojikato/bind/introduction/theme2.html

    • Related Report
      2013 Final Research Report
  • [Remarks] ワサビによる抗ガン・抗菌作用について

    • URL

      http://www.shse.u-hyogo.ac.jp/yojikato/bind/introduction/theme2.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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