Investigation of target proteins by isothiocyanate with fluorescent probe
| Project/Area Number |
24658127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Food science
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| Research Institution | University of Hyogo |
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Principal Investigator |
KATO Yoji 兵庫県立大学, 環境人間学部, 教授 (30305693)
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| Co-Investigator(Kenkyū-buntansha) |
中村 宜督 岡山大学, 環境生命科学研究科, 准教授 (60324381)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | イソチオシアネート / 蛍光 / アミノ付加体 / 標的タンパク質 / 生理機能 |
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| Research Abstract |
Though isothiocyanates have some biological functions such as anti-cancer or anti-bacterial activities, the mechanisms has not been shown in detail. We found the covalent adduction of ITC with lysine in a protein at physiological conditions. In this study, we focused in fluorescent character of ITC by using FITC. A monoclonal antibody to fluorescent structure has been prepared and characterized and then applied to fluorescent staining of cells. The multiple ITC adducted proteins were then detected in a gel by fluorescent scanning or by specific antibody. The identification of ITC adducted proteins remains to be done.
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Report
(3 results)
Research Products
(4 results)
