Development of universal transcriptome analysis method toward functional genomics in woody plants
| Project/Area Number |
24658130
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAITO Hideyuki 北海道大学, (連合)農学研究科(研究院), 講師 (70312395)
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| Co-Investigator(Kenkyū-buntansha) |
SESE Jun 東京工業大学, 情報理工学研究科, 准教授 (40361539)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 機能ゲノム学 / DNAマイクロアレイ / トランスクリプトーム / ドラフトゲノム / ブナ / ゲノム網羅的解析 / 遺伝子発現 / RNA-seq |
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| Research Abstract |
To advance the functional genomics of non-model woody plant, Fagus crenata (Fagaceae), an ecologically important tree species, two studies was conducted as follows; (1) DNA microarray created from a F. crenata cDNA sequence data-set is examined if versatile tool to study transcriptome comparing among intraspecific variations or not, and (2) F. crenata draft genome was generated. The transcriptome analysis by using the F. crenata DNA microarray was available for three populations, Kuromatsunai in Hokkaido Island, Tanzawa and Fuji in Honshu Island, suggesting the method is versatile in F. crenata. A draft genome of F. crenata was generated with 442 Mbp (including gap), allowing 48,618 predicted genes. This genomic information of F. crenata would contribute to accelerate the functional genomics of woody plants in family Fagaceae.
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Report
(3 results)
Research Products
(20 results)
