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Establishment of a method for synthesizing artificial Shiro of Tricholoma matsutake using split-root system

Research Project

Project/Area Number 24658131
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Forest science
Research InstitutionThe University of Tokyo

Principal Investigator

MATSUSHITA Norihisa  東京大学, 農学生命科学研究科, 准教授 (00282567)

Co-Investigator(Renkei-kenkyūsha) WU Bingyun  東京大学, 大学院農学生命科学研究科, 助教 (10396814)
Research Collaborator NATSUME Yuuki  東京大学, 大学院農学生命科学研究科, 修士2年
YOSHIDA Naohiro  東京大学, 大学院農学生命科学研究科, 博士1年
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsマツタケ / シロ / 外生菌根菌 / 分根 / 共生 / アカマツ / 外生菌根 / 根分け / クロマツ
Research Abstract

To produce fruiting bodies of Tricholoma matsutake under controlled conditions, it is necessary to develop Shiro which is compact mass of mycelia and mycorrhizae of T. matsutake. In this study, we tried to establish a method for synthesizing artificial Shiro using split-root system. We examined the moisture condition of the seedbed and the age of seedlings to be used for cuttings, and determined the optimal condition for obtaining the cuttings of Japanese red pine and Japanese black pine. In addition, we established the inoculation method of ectomycorrhizal fungi for the split-root system. It was possible to investigate the translocation of nitrogen from mycorrhizal root systems to nonmycorrhizal root systems using the split-root system of Japanese red pine. Therefore, we considered that the sprit-root system established in this study to be very effective in studying the ectomycorrhizal symbiosis.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 Other

All Presentation (6 results) (of which Invited: 1 results)

  • [Presentation] アカマツ挿し木分根培養系における窒素転流への施肥および外生菌根菌接種の影響2014

    • Author(s)
      棗悠紀・松下範久
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県)
    • Related Report
      2013 Final Research Report
  • [Presentation] アカマツ挿し木分根培養系における窒素転流への施肥および外生菌根菌接種の影響2014

    • Author(s)
      棗悠紀・松下範久
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マツタケ外生菌根共生系の生態2013

    • Author(s)
      松下範久
    • Organizer
      第1回マツタケ講演会
    • Place of Presentation
      徳島大学(徳島県)
    • Year and Date
      2013-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] マツタケのシロの拡大に伴う外生菌根菌相および土壌菌類相の変化2013

    • Author(s)
      松下範久・小松隆平・竹内嘉江・宝月岱造
    • Organizer
      第124回日本森林学会大会
    • Place of Presentation
      岩手大学(岩手県)
    • Related Report
      2013 Final Research Report
  • [Presentation] マツタケのシロの拡大に伴う外生菌根菌相および土壌菌類相の変化2013

    • Author(s)
      松下範久
    • Organizer
      第124回日本森林学会大会
    • Place of Presentation
      岩手大学(岩手県)
    • Related Report
      2012 Research-status Report
  • [Presentation] マツタケ外生菌根共生系の生態

    • Author(s)
      松下範久
    • Organizer
      第1回マツタケ講演会
    • Place of Presentation
      徳島大学工学部(徳島県)
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-31   Modified: 2019-07-29  

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