• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Estimation of the acutual gross nitrification rate in forest soils using stable isotope information

Research Project

Project/Area Number 24658133
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Forest science
Research InstitutionKyoto University (2014)
The University of Tokyo (2012-2013)

Principal Investigator

OHTE Nobuhito  京都大学, 情報学研究科, 教授 (10233199)

Co-Investigator(Kenkyū-buntansha) TOKUCHI Naoko  京都大学, フィールド科学教育研究センター, 教授 (60237071)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords森林生態系 / 窒素循環 / 安定同位体 / 硝化 / 総硝化量 / 不動化量 / 森林生態 / 生態系保全 / NO3- / 安定同位体比 / 同位体希釈法 / 森林生態・保護・保全
Outline of Final Research Achievements

The aim of this study was to elucidate the actual gross nitrification rate of forest soils using isotope tracer techniques, which could not be measured by conventional incubation approach. We proposed combined usage of the d18O of NO3- measurements of field water samples and the pool dilution method using 15N tracers. Experimental results at the University of Tokyo, Tanashi Forest showed that the d18O of NO3- decreased drastically at the organic litter layer and the surface part of mineral soil horizon. This indicated that the NO3- supplied by atmospheric deposition was rapidly immobilized and replaced by the NO3- produced by nitrifying microbes. The gross nitrification and immobilization rates measured using the pool dilution method evidenced the NO3- replacement processes consistently.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2015 2014 2013 2012

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (10 results) (of which Invited: 4 results) Book (1 results)

  • [Journal Article] Soil nitrogen transformation dynamics in a suburban forest near Tokyo Metropolitan Area under high nitrogen deposition: A case study using stable isotope tracer techniques2015

    • Author(s)
      Shi, J., N. Ohte, N. Tokuchi, K. Isobe, R. Urakawa, N. Imamura, T. Oda, and M. Suzuki
    • Journal Title

      Bulletin of University of Tokyo Forest

      Volume: 132 Pages: 17-34

    • NAID

      120005588096

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Microbial regulation of nitrogen dynamics along the hillslope of a natural forest2015

    • Author(s)
      Isobe, K., N. Ohte, T. Oda, S. Murabayashi, W. Wei, K. Senoo, N. Tokuchi, and R. Tateno
    • Journal Title

      Frontiers in Environmental Science

      Volume: 2 Pages: 1-8

    • DOI

      10.3389/fenvs.2014.00063

    • NAID

      120005946998

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Higher diversity and abundance of denitrifying microorganisms in environments than considered previously2015

    • Author(s)
      Wei, W., K. Isobe, T. Nishizawa, L. Zhu, Y. Shiratori, N. Ohte, K. Koba, S. Otsuka, and K. Senoo
    • Journal Title

      The ISME Journal

      Volume: 10 March 2015 Issue: 9 Pages: 1-12

    • DOI

      10.1038/ismej.2015.9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Nitrate isotopic composition reveals nitrogen deposition and transformation dynamics along the canopy–soil continuum of a suburban forest in Japan2014

    • Author(s)
      Shi, J., N. Ohte, N. Tokuchi, N. Imamura, M. Nagayama, T. Oda, and M. Suzuki
    • Journal Title

      Rapid Communication in Mass Spectrometry

      Volume: 28 Issue: 23 Pages: 2539-2549

    • DOI

      10.1002/rcm.7050

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Seasonal changes and controlling factors of gross N transformation in an evergreen plantation forest in central Japan2014

    • Author(s)
      Tokuchi, N., S. Yoneda, N. Ohte, N. Usui, K. Koba, M. Kuroiwa, H. Toda, and Y. Suwa
    • Journal Title

      Journal of Forest Research

      Volume: 19 Issue: 1 Pages: 77-85

    • DOI

      10.1007/s10310-013-0391-4

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ecological perspectives on microbes involved in N cycling2014

    • Author(s)
      Isobe, K. and N. Ohte
    • Journal Title

      Microbes and Environments

      Volume: in press

    • Related Report
      2013 Research-status Report
  • [Journal Article] Separate estimation of N export into baseline N leakage without disturbance and N loss due to insect defoliation in a pine forest watershed in central Japan2013

    • Author(s)
      Tokuchi, N., N. Ohte, K. i. Osaka, and M. Katsuyama
    • Journal Title

      Environmental Monitoring and Assessment

      Volume: 185 Issue: 1 Pages: 855-863

    • DOI

      10.1007/s10661-012-2596-y

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tracing sources and pathways of dissolved nitrate in forest and river ecosystems using high-resolution isotopic techniques: a review2012

    • Author(s)
      Ohte, N.
    • Journal Title

      Ecological Research

      Volume: Online first Issue: 5 Pages: 1-9

    • DOI

      10.1007/s11284-012-0939-3

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Implications of seasonal variation in nitrate export from forested ecosystems: a review from the hydrological perspective of ecosystem dynamics2012

    • Author(s)
      Ohte, N.
    • Journal Title

      Ecological Research

      Volume: 27 Issue: 4 Pages: 657-665

    • DOI

      10.1007/s11284-012-0956-2

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Nitrogen Deposition and Transformation Dynamics along the canopy–soil Continuum of a Suburban Forest in Japan2015

    • Author(s)
      Shi J, Ohte N, Tokuchi N, Imamura N, Nagayama M, Oda T & Suzuki M
    • Organizer
      GoldSchmidt 2015
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2015-08-16 – 2015-08-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Relevant approaches and strategies for investigation on the nitrogen saturation in forested catchments2015

    • Author(s)
      N. Ohte, K. Isobe and N. Tokuchi
    • Organizer
      Japan Geoscience Union
    • Place of Presentation
      幕張メッセ、千葉県千葉市
    • Year and Date
      2015-05-24 – 2015-05-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Consideration of N dynamics in the forest ecosystem related with the anthropogenic N deposition, other element including carbon, water and environmental change2014

    • Author(s)
      Naoko Tokuchi
    • Organizer
      The 6th EAFES International Congress
    • Place of Presentation
      Haikou, China
    • Related Report
      2013 Research-status Report
  • [Presentation] Close relationship between nitrogen dynamics and microbial communities in forest soil2014

    • Author(s)
      Kazuo Isobe, Nobuhito Ohte, Hiroyu Kato, Tomoki Oda, Ryunosuke Tateno
    • Organizer
      The 6th EAFES International Congress
    • Place of Presentation
      Haikou, China
    • Related Report
      2013 Research-status Report
  • [Presentation] 森林集水域における窒素循環研究の今日的な課題とそれに対するストラテジー2013

    • Author(s)
      大手信人、徳地直子
    • Organizer
      2013年度日本水文科学会学術大会
    • Place of Presentation
      千葉
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Strong nitrogen retention against the pinestands dieback by pinewilt disease in a temperate coniferdeciduous forest in central Japan: Budget and mechanisms2013

    • Author(s)
      Nobuhito Ohte, Naoko Tokuchi, Masanori Katsuyama, Ken'ichi Osaka, Satomi Yoneda, Natsuhito Ochiai
    • Organizer
      American Geophysical Union, Fall Meeting 2013
    • Place of Presentation
      San Francisco
    • Related Report
      2013 Research-status Report
  • [Presentation] Spatial distribution of nitrate sources of rivers in the Lake Biwa watershed, Japan: Controlling factors revealed by nitrogen and oxygen isotopes of nitrate2013

    • Author(s)
      NOBUHITO OHTE, CHIKAGE YOSHIMIZU, AYATO KOHZU, ICHIRO TAYASU, KEN’ICHI OSKA and TOSHI NAGATA
    • Organizer
      US-Japan Joint Seminar on Responses of Catchment Hydrology and Forest Biogeochemistry to Climatic and Environmental Change
    • Place of Presentation
      Honolulu, Hawai'i, USA
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Whole story of episodic high nitrate discharge due to the partial dieback of a pine forest in Japan: runoff generation processes control seasonality2013

    • Author(s)
      Nobuhito Ohte, Naoko Tokuchi, Masanori Katsuyama, Ken’ichi Osaka
    • Organizer
      US-Japan Joint Seminar on Responses of Catchment Hydrology and Forest Biogeochemistry to Climatic and Environmental Change
    • Place of Presentation
      Honolulu, Hawai'i, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 森林集水域における窒素循環研究の課題とストラテジー2013

    • Author(s)
      大手信人・徳地直子
    • Organizer
      第124回日本森林学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Sources and processes of nitrate in forest soils: a case study at sites with heavy nitrogen deposition using nitrogen and oxygen isotopes2012

    • Author(s)
      Shi Jun, Nobuhito Ohte, Naohiro Imamura, Tomoki Oda and Miyuki Nagayama
    • Organizer
      Japan Geoscience Union Meeting
    • Place of Presentation
      Chiba
    • Related Report
      2012 Research-status Report
  • [Book] 物質循環研究の今後の展開 『森のバランス: 植物と土壌の相互作用』2012

    • Author(s)
      徳地直子・大手信人
    • Total Pages
      300
    • Publisher
      東海大学出版会
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi