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A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing

Research Project

Project/Area Number 24658138
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Forest science
Research InstitutionKyushu University

Principal Investigator

SHIRAISHI Susumu  九州大学, 大学院・農学研究院, 教授 (70226314)

Project Period (FY) 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
KeywordsDNAマーカー / マイクロサテライト / 4塩基繰返し / 森林科学 / 育種学 / 分子生態学 / 4塩基繰返し
Research Abstract

DNA molecular markers were widely utilized as powerful tool in breeding, genetics, and ecology. Among these markers, microsatellite marker is highly variable, and possesses a large quantity of genetic information. Constructing a new system, a next generation sequencer (pyrosequencing) was introduced to gain the huge sequences of microsatellite regions in genomes. The new system showed the possibility for a simple and inexpensive microsatellite development.

Report

(2 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (4 results)

All 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Development of tetra-nucleotide microsatellite markers in Pinus kesiyaRoyle ex Gordon2013

    • Author(s)
      Thao DV, Yamashita M, Watanabe A, Shiraishi S
    • Journal Title

      Conservation Genet Resour

      Volume: 5 Pages: 405-407

    • Related Report
      2012 Final Research Report
  • [Journal Article] Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii2013

    • Author(s)
      Thao DV, Widyatmoko AYPBC, Guan L Watanabe A, Gotoh E, Shiraishi S
    • Journal Title

      Conservation Genet Resour

      Volume: 5 Pages: 433-436

    • Related Report
      2012 Final Research Report
  • [Journal Article] Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon2012

    • Author(s)
      D. V. Thao, M. Yamashita, A. Watanabe, S. Shiraishi
    • Journal Title

      Conservation Genetics Resources

      Volume: 5 Issue: 2 Pages: 405-407

    • DOI

      10.1007/s12686-012-9814-3

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii2012

    • Author(s)
      D. V. Thao, A. Y. P. B. C. Widyatmoko, L. Guan, E. Gotoh, A. Watanabe, S. Shiraishi
    • Journal Title

      Conservation Genetics Resources

      Volume: 5 Issue: 2 Pages: 433-436

    • DOI

      10.1007/s12686-012-9821-4

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed

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Published: 2013-05-31   Modified: 2019-07-29  

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