Utilization of entomophilic nematodes as carriers of entomopathogenic microbes

• Project/Area Number: 24658150
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: KANZAKI Natsumi 独立行政法人森林総合研究所, 森林微生物研究領域, 主任研究員 (70435585)
• Co-Investigator(Kenkyū-buntansha): MAEHARA Noritoshi 森林総合研究所, 東北支所, 主任研究員 (20343808)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生物防除 / 天敵微生物 / 昆虫嗜好性線虫 / 昆虫 / マツノマダラカミキリ / 昆虫病原糸状菌 / 微生物生態学

Research Abstract

The nematode fauna associated with the Japanese pine sawyer, Monochamus alernatus was examined. In total, six species on nematodes including pine wood nematode, Bursaphelenhcus xylophilus and Contortylenchus genitalicala were isolated. Within these nematode species, undescribed Diplogasteroides sp. was considered as a candidate for the carrier of entomopathogenic microbes. The nematode species was then, described as D. andrassyi n. sp., and feeding experiment was conducted, i.e., the nematode's ability to carry the microbes were examined. The experiment suggested that the nematode species which has wider stoma can be more effective carrier than D. andrassyi.

Research Products

• [Journal Article] Description of Diplogasteroides andrassyi n. sp., associated with Monochamus grandis and Pinaceae trees in Japan (2013)
  • Author(s): Kanzaki, N., Tanaka, R. Hirooka, Y. & Maehara, N.
  • Journal Title: Journal of Nematode Morphology and Systematics Volume: 16 Pages: 35-47
  • Peer Reviewed
• [Journal Article] An entomoparasitic adult form in Bursaphelenchus doui (Nematoda : Tylenchomorpha) associated with Acalolepta fraudatrix (2013)
  • Author(s): Kanzaki, N., Maehara, N., Aikawa, T. & Nakamura, K.
  • Journal Title: Journal of Parasitology Volume: 99 Pages: 803-815
  • Peer Reviewed
• [Journal Article] Two androdioecious and one dioecious new species of Pristionchus (Nematoda : Diplogastridae): new reference points for the evolution of reproductive mode (2013)
  • Author(s): Kanzaki, N., Ragsdale, E.J., Herrmann, M., Sommer, R.J. & Susoy, V.
  • Journal Title: Journal of Nematology Volume: 45 Pages: 172-194
  • Peer Reviewed
• [Journal Article] Effects of two species of cerambycid beetles, tribe Lamiini (Coleoptera : Cerambycidae), on the phoretic stage formation of two species of nematodes, genus Bursaphelenchus (Nematoda : Aphelenchoididae) (2013)
  • Author(s): Maehara, N., Kanzaki, N., Aikawa, T. & Nakamura, K.
  • Journal Title: Nematological Research Volume: 43 Pages: 9-13
  • NAID: 130004837322
  • Peer Reviewed
• [Journal Article] Description of Diplogasteroides andrassyi n. sp., associated with Monochamus grandis and Pinaceae trees in Japan. (2013)
  • Author(s): Kanzaki, N., Tanaka, R. Hirooka, Y. & Maehara, N.
  • Journal Title: Journal of Nematode Morphology and Systematics Volume: 16 Pages: 35-47
  • Peer Reviewed
• [Journal Article] An entomoparasitic adult form in Bursaphelenchus doui (Nematoda: Tylenchomorpha) associated with Acalolepta fraudatrix. (2013)
  • Author(s): Kanzaki, N., Maehara, N., Aikawa, T. & Nakamura, K.
  • Journal Title: Journal of Parasitology Volume: 99 Pages: 803-815
  • Peer Reviewed
• [Journal Article] Description of three Pristionchus species (Nematoda: Diplogastridae) from Japan that form a cryptic species complex with the model organism P. pacificus. (2012)
  • Author(s): Kanzaki, N., Ragsdale, E.J., Herrmann, M., Mayer, W. E. & Sommer, R. J
  • Journal Title: Zoological Science Volume: 29 Issue: 6 Pages: 403-417
  • DOI: 10.2108/zsj.29.403
  • NAID: 40019296510
  • Peer Reviewed
• [Journal Article] Two new species of Pristionchus (Rhabditida: Diplogastridae): P. fissidentatus n. sp. from Nepal and La Réunion Island and P. elegans n. sp. from Japan. (2012)
  • Author(s): Kanzaki, N., Ragsdale, E.J., Herrmann, M. & Sommer, R. J.
  • Journal Title: Journal of Nematology Volume: 44 Pages: 80-91
  • Peer Reviewed
• [Presentation] Amensalistic relationship between Bursaphelenchus sp. and a Japanese native weevil, Niphades variegatus (2013)
  • Author(s): Tanaka, S.E., Tanaka, R., Akiba, M., Takeuchi, Y. & Kanzaki, N.
  • Organizer: Society of Nematologists 52nd Annual Meeting
  • Place of Presentation: Hilton Knoxville, Knoxville, TN
• [Presentation] Amensalistic relationship between Bursaphelenchus sp. and a Japanese native weevil, Niphades variegatus. (2013)
  • Author(s): Tanaka, S.E., Tanaka, R., Akiba, M., Takeuchi, Y. & Kanzaki, N.
  • Organizer: Society of Nematologists 52nd Annual Meeting.
  • Place of Presentation: Hilton Knoxville, Knoxville, TN.