| Project/Area Number |
24658161
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Wood science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
TAKANO Mariko 独立行政法人森林総合研究所, きのこ・微生物研究領域, 主任研究員 (10353749)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 菌糸鞘 / 白色腐朽菌 / リグニン / ペルオキシダーゼ |
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| Research Abstract |
White-rot fungus was cultivated in ligninolytic conditions and was histochemically analyzed distribution of lignin-degrading enzyme activity and hyphal sheath. Lignin-degrading enzyme reaction was observed as peroxidase activity staining at hyphal tips after 2-3 days cultivation and was observed to extended in hyphal sheath after 3-4 days of cultivation. It was confirmed that the enzyme, which produced peroxidase activity staining, is manganese peroxidase. N-terminal amino acid sequence of the enzyme protein was consistent with manganese peroxidase gene sequences. These results showed that the manganese peroxidase reaction occurred in hyphal sheath of a white rot fungus, and suggested that hyphal sheath of white-rot fungus functioned as a lignin-degrading bioreactor.
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