Epigenetics of the rotifer Brachionus plicatilis and its use as experimental animal
| Project/Area Number |
24658174
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Nagasaki University |
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Principal Investigator |
HAGIWARA Atsushi 長崎大学, 水産・環境科学総合研究科(水産), 教授 (50208419)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGA Koushirou 長崎大学, 水産・環境科学総合研究科(水産), 准教授 (60569185)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 水産学 / シオミズツボワムシ / エピジェネティクス / 実験動物 / ワムシ / 両性生殖 / 遺伝様式 / 個体群動態 / 国際情報交換(韓国） / 国際情報交換（韓国） / 国際情報交換（米国） / 国際情報交換（スペイン） |
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| Outline of Final Research Achievements |
Starvation treatment to maternal rotifers resulted in the rise in mixis induction of Brachionus manjavacas, and it inherited up to the 38th generation. This may be explained by epigenetic inheritance involving methylation of the DNA. A primer was designed using partial base sequence of the methyl transferase (DNA methylase). As a result of templating cDNA of B. manjavacas, and having performed PCR with the primer designed on base sequence of Brachionus plicatilis s.s., the amplification of the DNA fragment of predicted size was confirmed.
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Report
(4 results)
Research Products
(4 results)
