| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Starvation treatment to maternal rotifers resulted in the rise in mixis induction of Brachionus manjavacas, and it inherited up to the 38th generation. This may be explained by epigenetic inheritance involving methylation of the DNA. A primer was designed using partial base sequence of the methyl transferase (DNA methylase). As a result of templating cDNA of B. manjavacas, and having performed PCR with the primer designed on base sequence of Brachionus plicatilis s.s., the amplification of the DNA fragment of predicted size was confirmed.
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