Snap-shot observation methods for natural aquatic microorganisms
| Project/Area Number |
24658180
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Fisheries Research Agency |
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Principal Investigator |
NAGASAKI Keizo 独立行政法人水産総合研究センター, 研究推進部, 研究開発コーディネーター (00222175)
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| Project Period (FY) |
2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 透過型電子顕微鏡 / 植物プランクトン / 凍結置換法 / 赤潮 / 微細藻類 / 水圏微生物生態学 / 超低温凍結固定 |
|---|
| Research Abstract |
The objective of the present study is to establish a novel method for TEM observation of microalgae by using the “rapid freezing and freeze substitution” techniques. By using a portable deep freezer and half-frozen acetone, several strains of microalga were nicely prepared for TEM observation. The new technique is introduced at a website for its dissemination: (http://feis.fra.affrc.go.jp/keisou_Virus/kimura_freeze.html).
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Report
(2 results)
Research Products
(6 results)
