Enzymatic asymmetric cleavage of aquatic products-derived carotenoids and the bio-modulating mechanism of the enzymolysis products

• Project/Area Number: 24658184
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Fisheries chemistry
• Research Institution: Shijonawate Gakuen University (2013); Kyoto University (2012)
• Principal Investigator: HIRATA Takashi 四條畷学園大学, リハビリテーション学部, 教授 (40273495)
• Co-Investigator(Kenkyū-buntansha): SUGAWARA Tatsuya 京都大学, 大学院農学研究科, 教授 (70378818)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 酵素分解 / 機能性 / カロテノイド / 機能性成分

Research Abstract

CMO2, betaCarotene 9, 10 monooxygenase, exhibits wide substrate specificity, and it can metabolize xanthophyll as well as betacarotene. We investigated whether CMO2 could cleave aquatic products derived carotenoids through evaluating CMO2 activity in mouse liver homogenate, the activity of recombinant CMO2, and CMO2 activity using CMO2 expressing cultured cells. We, however, could not detect the CMO2 activity in mouse liver homogenate. Although recombinant human CMO2 was produced by Baculotechnologies Company Limited, enzymatically active CMO2 was not obtained. According to a previous report, MDA MB 231, human breast cancer cell line, expresses CMO2, and then, we used this cell line in order to evaluate the CMO2 activity. We could detect no CMO2 metabolites accumulated in carotenoid treated MDA MB 231.

Research Products

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  • URL: http://www.bioproducts.marine.kais.kyoto-u.ac.jp/