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Instability of proteins from aquatic animals as revealed by molecular dynamics simulation

Research Project

Project/Area Number 24658186
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionTokai University

Principal Investigator

OCHIAI Yoshihiro  東海大学, 海洋学部, 教授 (50160891)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsタンパク質 / 構造揺らぎ / 不安定性 / 魚介類 / 分子シミュレーション / 筋肉 / 安定性
Research Abstract

Molecular dynamics simulation was applied to the mechanism of instability of proteins (namely, tropomyosin, myoglobin and myosin) from some aquatic organisms. The extent of structural fluctuations under varied temperature and pressured conditions were examined in detail. Regarding the two proteins except myosin, the regions responsible for the instability could be identified. Especially in case of tropomyosin, the effects of amino acid substitutions on the bends in the molcule could be addressed. On the other hand, in the case of myoglobin, the detaied study revealed the extent of fluctuation under the wide range of temperature and pressured conditions, which might be a possible factor for the molecular adaptation of each animal species to the respective habitat.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 Other

All Journal Article (1 results) Presentation (10 results)

  • [Journal Article] ミオグロビン―基礎から応用まで2012

    • Author(s)
      落合芳博
    • Journal Title

      冷凍

      Volume: 87 Pages: 341-347

    • NAID

      10030763746

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Strategies of acclimation to deep sea : Structure simulation of myoglobin molecules from aquatic animals under high pressure2013

    • Author(s)
      Y. Ochiai, H. Ozawa
    • Organizer
      10^<th> International Marine Biotechnology Conference
    • Place of Presentation
      Brisbane Convention & Exhibition Centre, Australia
    • Year and Date
      2013-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of flexibility and curvature of tropomyosin by molecular dynamics simulation2013

    • Author(s)
      H. Ozawa, Y. Ochiai, K. Umezawa, S. Ishiwata, M. Takano
    • Organizer
      日本生物物理学会年会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2013-10-29
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 分子シミュレーションを用いた筋肉トロポミオシン断片の局所構造解析2013

    • Author(s)
      小澤秀夫、梅澤公二、高野光則、石渡信一、渡部終五、落合芳博
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      三重大学生物資源学部
    • Year and Date
      2013-09-21
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Simulation of the Thermal-stability differences of myoglobins from aquatic animals2013

    • Author(s)
      Y. Ochiai
    • Organizer
      Asian Biophysics Association
    • Place of Presentation
      Ramada Plaza, Jeju (韓国)
    • Year and Date
      2013-05-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 水産分野における分子シミュレーションの有用性2012

    • Author(s)
      落合芳博
    • Organizer
      日本水産学会中部支部大会
    • Place of Presentation
      アスト津(津市)
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] MD simulation of the structural fluctuations of sea hare Aplysia limacina myoglobin2012

    • Author(s)
      Y. Ochiai
    • Organizer
      The 9th Asia-Pacific Marine Biotechnology Conference
    • Place of Presentation
      カルポート(高知)
    • Year and Date
      2012-07-13
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] Simulation of the thermal-stability differences of myoglobin from aquatic animals

    • Author(s)
      Y. Ochiai
    • Organizer
      8th Asian Biophysics Association Symposium
    • Place of Presentation
      Ramada Hotel, Jeju, Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] Strategies of acclimation to deep sea: Structure simulation of myoglobin molecules from aquatic animals under high pressure

    • Author(s)
      Y. Ochiai, H. Ozawa
    • Organizer
      10th International Marine Biotechnology Conference
    • Place of Presentation
      Brisbane Convention & Exhibition Centre, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] 水産分野における分子シミュレーションの有用性

    • Author(s)
      落合芳博
    • Organizer
      日本水産学会中部支部大会
    • Place of Presentation
      アスト津(津市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Simulation of the Thermal-stability Differences of Myoglobins from Aquatic Animals

    • Author(s)
      Y. Ochiai
    • Organizer
      Asian Biophysics Association Jeju 2013
    • Place of Presentation
      Ramada Plaza Jeju(韓国)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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