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Elusidation of microstructure of biofilm attached on the surface of agricultural produce

Research Project

Project/Area Number 24658212
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Agricultural environmental engineering
Research InstitutionKyushu University

Principal Investigator

UCHINO Toshitaka  九州大学, (連合)農学研究科(研究院), 教授 (70134393)

Co-Investigator(Kenkyū-buntansha) TANAKA Fumihiko  九州大学, 大学院農学研究院, 准教授 (30284912)
HAMANAKA Daisuke  九州大学, 大学院農学研究院, 助教 (60399095)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsバイオフィルム / 共焦点レーザー顕微鏡 / 細胞外多糖類 / 乳製品副産物 / インデンテーション法 / 微細構造
Research Abstract

An effect of dairy byproducts on adherence of dairy related bacteria to a stainless steel coupon surface conditioned by the byproducts was evaluated. The layer formed by skim milk buttermilk butter serum was thinnest, medium and thickest, respectively. Three dimensional images of biofilm were obtained by using a confocal laser scanning microscopy. In the result the conditioning by the byproducts controlled the bacterial adherence.
A hardness of biofilm was determined by indentation method. Twenty micro m of loading and 10 micro m of unloading by a universal material testing machine were repeated until a plunger head touching a plastic coupon. Thickness of biofilm was calculated from a length between contact points of the plunger head with the coupon and biofilm. The biofilm strength increased with decrease of biofilm thickness.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Surface conditioning of stainless steel coupons with skim milk, buttermilk, and butter serum solutions and its effect on bacterial adherence2014

    • Author(s)
      M. D. Nguyen, L.D. Manh, D. Hamanaka, H. V. Duong, F. Tanaka, T. Uchino
    • Journal Title

      Food Control

      Volume: 42 Pages: 94-100

    • DOI

      10.1016/j.foodcont.2014.01.040

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] インデンテーション法によるバイオフィルムの力学特性評価の可能性2013

    • Author(s)
      江口広大,堀海,森本愛子,濱中大介,金山素平,田中史彦,内野敏剛
    • Journal Title

      農業食料工学会九州支部誌

      Volume: 62 Pages: 1-5

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] インデンテーション法によるバイオフィルムの力学特性評価の可能性2013

    • Author(s)
      江口広大,堀海,森本愛子,濱中大介,金山素平,田中史彦,内野敏剛
    • Organizer
      農業食料工学会第72回年次大会
    • Place of Presentation
      帯広市
    • Related Report
      2013 Final Research Report
  • [Presentation] Influence of some dairy by-products on the adherence of Lactococcus lactis2013

    • Author(s)
      M. D. Nguyen, L.D. Manh, D. Hamanaka, H. V. Duong, F. Tanaka, T. Uchino
    • Organizer
      Int. WS on Agric. Eng. & Post-harvest Tech. for Asia Sustainability
    • Place of Presentation
      Hanoi, Vietnam
    • Related Report
      2013 Final Research Report
  • [Presentation] インデンテーション法によるバイオフィルムの力学特性評価の可能性2013

    • Author(s)
      江口広大,堀海,森本愛子,濱中大介,金山素平,田中史彦,内野敏剛
    • Organizer
      農業食料工学会第72回年次大会
    • Place of Presentation
      帯広畜産大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Influence of some dairy by-products on the adherence of Lactococcus lactis2013

    • Author(s)
      Nguyen Manh Dat, Le Duc Manha, Daisuke Hamanaka, Duong Van Hung, Fumihiko Tanaka, Toshitaka Uchino
    • Organizer
      Int. WS on Agric. Eng. & Post-harvest Tec. for Asia Sustanability
    • Place of Presentation
      Hoa Binh Hotel, Hanoi, Vietnam
    • Related Report
      2013 Annual Research Report
  • [Presentation] バイオフィルムを形成したPseudomonas putidaへの洗浄効果2012

    • Author(s)
      江口広大,内野敏剛,田中史彦,濱中大介,森松和也
    • Organizer
      第66回農業機械学会九州支部例会
    • Place of Presentation
      宮崎市
    • Related Report
      2013 Final Research Report
  • [Presentation] バイオフィルムを形成したPseudomonas putidaへの洗浄効果2012

    • Author(s)
      江口広大
    • Organizer
      第66回農業機械学会九州支部例会
    • Place of Presentation
      宮崎県サンホテルフェニックス
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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