| Project/Area Number |
24658224
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Tohoku University |
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Principal Investigator |
ASO Hisashi 東北大学, (連合)農学研究科(研究院), 教授 (50241625)
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| Co-Investigator(Kenkyū-buntansha) |
KITAZAWA Haruki 東北大学, 大学院農学研究科, 准教授 (10204885)
WATANABE Kouichi 東北大学, 大学院農学研究科, 助教 (80261494)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 乳房炎 / シクロフィリンA / 遊走因子 / 診断法 / 炎症 / 乳汁 / CyPA / 乳腺上皮細胞 / イムノクロマト / 簡便 / 泌乳期 / 慢性乳房炎 |
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| Research Abstract |
Cyclophilin A (CyPA), a member of the peptidyl-prolyl cis-trans isomerase family, is known as a cyclosporin binding-protein. Although CyPA was initially believed to function primarily as an intracellular protein, recent studies have revealed that it can be secreted by cells in response to inflammatory stimuli. Therefore, CyPA may play a role in cardiovascular diseases, viral infections and others. Further, extracellular CyPA has a potent chemotactic effect on leukocytes, monocytes, and lymphocytes through CD147 cell receptor.
We investigated the expression of CyPA in bovine mammary gland. CyPA was detected in mammary epithelial cells of mastitis cow stronger than healthy cow. Surprisingly, CyPA in milk was stained in mammary gland alveolus of mastitis cow with inflammation of leukocytes, but not in healthy cow. The content of CyPA in milk was greater in mastitis cow than healthy cow. These data indicate that CyPA in milk may be a marker of mastitis.
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