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Cyclophilin A is a early stage maker of bovine mastitis.

Research Project

Project/Area Number 24658224
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Zootechnical science/Grassland science
Research InstitutionTohoku University

Principal Investigator

ASO Hisashi  東北大学, (連合)農学研究科(研究院), 教授 (50241625)

Co-Investigator(Kenkyū-buntansha) KITAZAWA Haruki  東北大学, 大学院農学研究科, 准教授 (10204885)
WATANABE Kouichi  東北大学, 大学院農学研究科, 助教 (80261494)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords乳房炎 / シクロフィリンA / 遊走因子 / 診断法 / 炎症 / 乳汁 / CyPA / 乳腺上皮細胞 / イムノクロマト / 簡便 / 泌乳期 / 慢性乳房炎
Research Abstract

Cyclophilin A (CyPA), a member of the peptidyl-prolyl cis-trans isomerase family, is known as a cyclosporin binding-protein. Although CyPA was initially believed to function primarily as an intracellular protein, recent studies have revealed that it can be secreted by cells in response to inflammatory stimuli. Therefore, CyPA may play a role in cardiovascular diseases, viral infections and others. Further, extracellular CyPA has a potent chemotactic effect on leukocytes, monocytes, and lymphocytes through CD147 cell receptor.
We investigated the expression of CyPA in bovine mammary gland. CyPA was detected in mammary epithelial cells of mastitis cow stronger than healthy cow. Surprisingly, CyPA in milk was stained in mammary gland alveolus of mastitis cow with inflammation of leukocytes, but not in healthy cow. The content of CyPA in milk was greater in mastitis cow than healthy cow. These data indicate that CyPA in milk may be a marker of mastitis.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (44 results)

All 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 1 results) Presentation (26 results) Book (4 results) Remarks (3 results)

  • [Journal Article] Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double muscled (DM) and normal muscled (NM) Japanese shorthorn cattle.2014

    • Author(s)
      H. Takahashi, K. Sato, T. Yamaguchi, M. Miyake, H. Watanabe, Y. Nagasawa, E. Kitagawa, S. Terada, M. Urakawa, M. T. Rose, C. D. McMahon, K. Watanabe, S. Ohwada, T. Gotoh, H. Aso
    • Journal Title

      Domestic Animal Endocrinology

      Volume: 48 Pages: 62-68

    • DOI

      10.1016/j.domaniend.2014.01.007

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of peripheral 5-HT on glucose and lipid metabolism in wether sheep.2014

    • Author(s)
      Watanabe H., Kitazawa H., Watanabe K., Aso H. 他
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 2 Pages: 1-12

    • DOI

      10.1371/journal.pone.0088058

    • NAID

      120005435471

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunoregulatory Effect of Bifidobacteria Strains in Porcine Intestinal Epithelial Cells through Modulation of Ubiquitin-Editing Enzyme A20 Expression.2013

    • Author(s)
      Tomosada, Y., H. Kitazawa, et al.
    • Journal Title

      PLoS ONE

      Volume: 8(3) Issue: 3 Pages: 1-12

    • DOI

      10.1371/journal.pone.0059259

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bifidobacteria Upregulate Expression of Toll-Like Receptor Negative Regulators Counteracting Enterotoxigenic Escherichia coli Mediated Inflammation in Bovine Intestinal Epitheliocytes.2013

    • Author(s)
      Murata K., Aso H., Kitazawa H., 他
    • Journal Title

      Open Journal of Veterinary Medicine

      Volume: 3 Issue: 02 Pages: 143-155

    • DOI

      10.4236/ojvm.2013.32023

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation.2013

    • Author(s)
      Takanashi, N. et al.
    • Journal Title

      BMC Microbiol.

      Volume: 13(54) Issue: 1 Pages: 1-16

    • DOI

      10.1186/1471-2180-13-54

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of myostatin in neural cells of the olfactory system.2012

    • Author(s)
      Syunsuke Iwasaki
    • Journal Title

      Molecular Neurobiology

      Volume: 47 Issue: 1 Pages: 1-8

    • DOI

      10.1007/s12035-012-8342-1

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in cattle.2012

    • Author(s)
      Masato Miyake
    • Journal Title

      Cell and Tissue Research

      Volume: 349 Issue: 2 Pages: 615-623

    • DOI

      10.1007/s00441-012-1422-8

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunobiotic Lactobacillus jensenii Modulates the Toll-Like Receptor 4-Induced Inflammatory Response via Negative Regulation in Porcine Antigen-Presenting Cells2012

    • Author(s)
      Villena, J., H.Kitazawa, et al
    • Journal Title

      Clin.Vac.Immunol.

      Volume: (未定)(印刷中) Issue: 7 Pages: 1038-1053

    • DOI

      10.1128/cvi.00199-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle.2012

    • Author(s)
      Chiba E., 他
    • Journal Title

      Research in Veterinary Science

      Volume: 93 Issue: 2 Pages: 688-694

    • DOI

      10.1016/j.rvsc.2011.10.002

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of a Lactobacilli, Oligosaccharide and Organic Germanium Intake on the Immune Responses of Mice2012

    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 2 Pages: 375-377

    • DOI

      10.1271/bbb.110655

    • NAID

      10030400214

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway2012

    • Author(s)
      Shimazu, T., H.Aso, Y.Suda, Y.Kawai, T.Saito, H.Kitazawa, et al
    • Journal Title

      Infect.Immun.

      Volume: 80 Issue: 1 Pages: 276-288

    • DOI

      10.1128/iai.05729-11

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 乳房炎牛乳汁中に増量するシクロフィリンAの細胞遊走活性2014

    • Author(s)
      高梨暁
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば市、国際会館
    • Year and Date
      2014-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳房炎多発症牛への枯草菌給与における血中樹状細胞の動態2013

    • Author(s)
      浦川めぐみ
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟市、新潟大学
    • Year and Date
      2013-09-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳房炎発症牛への枯草菌給与における栄養因子への影響2013

    • Author(s)
      佐藤勝祥
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟市、新潟大学
    • Year and Date
      2013-09-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳房炎発症牛への枯草菌給与における分娩後の栄養因子およびストレス指標の変動2013

    • Author(s)
      佐藤勝祥
    • Organizer
      第63回東北畜産学会大会
    • Place of Presentation
      山形市、山形テルサ
    • Year and Date
      2013-08-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳房炎多発症牛への枯草菌給与による乳房炎発症予防と血中樹状細胞の動態2013

    • Author(s)
      浦川めぐみ
    • Organizer
      第63回東北畜産学会大会
    • Place of Presentation
      山形市、山形テルサ
    • Year and Date
      2013-08-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 腸管M細胞における解糖系酵素アルドラーゼAを介したプリオン蛋白質取り込み機構2013

    • Author(s)
      長津裕哉
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      名古屋、安田女子大学
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 炎症性腸疾患における腸上皮細胞由来シクロフィリンAの作用解明2013

    • Author(s)
      染谷俊輔
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      名古屋、安田女子大学
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 炎症性腸疾患における腸上皮細胞由来シクロフィリンA の作用解明2013

    • Author(s)
      染谷俊輔・長澤裕哉・渡辺一史・菅原静夏・板谷奈波・渡邊康一・麻生久
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      名古屋、安田女子大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 腸管M 細胞における解糖系酵素アルドラーゼA を介したプリオン蛋白質取り込み機構2013

    • Author(s)
      長澤裕哉・高橋遊・本堂哲也・渡邉一史・寺田俊介・染谷俊輔・渡邊康一・大和田修一・北澤春樹・今村守一・横山隆・毛利資郎・麻生久
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      名古屋、安田女子大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Role of aldolase A in transcytosis of intestinal M cells2012

    • Author(s)
      Nagasawa Y.
    • Organizer
      2012 ASCB Annual Meeting. The American Society for Cell Biology
    • Place of Presentation
      San Francisco,USA
    • Year and Date
      2012-12-17
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳汁中リンパ球遊走因子シクロフィリンAの乳房炎早期診断マーカーへの応用2012

    • Author(s)
      麻生久
    • Organizer
      第17回日本乳房炎研究会学術集会
    • Place of Presentation
      南青山会館、東京
    • Year and Date
      2012-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 腸管M細胞における解糖系酵素アルドラーゼAを介した抗原取り込み機構2012

    • Author(s)
      長津裕哉
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学
    • Year and Date
      2012-09-14
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスOSS誘導大腸炎と白血球遊走因子シクロフィリンAとの関連性2012

    • Author(s)
      染谷俊輔
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学
    • Year and Date
      2012-09-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 高抗病性ランドレース豚の免疫担当臓器におけるサイトカイン発現の特性解析2012

    • Author(s)
      菅原静夏
    • Organizer
      第62回東北畜産学会大会
    • Place of Presentation
      秋田県立大学
    • Year and Date
      2012-08-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Prion protein binding proteins of bovine intestine M cell2012

    • Author(s)
      Nagasawa Y.
    • Organizer
      Asian Pacific Prion Symposium 2012
    • Place of Presentation
      Yokohama,Japan
    • Year and Date
      2012-07-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Role of aldolase A in transcytosis of intestinal M cells.2012

    • Author(s)
      Nagasawa Y., Takahashi Y., Hondo T., Watanabe H., Terada S., Someya S., Watanabe K., Ohwada S., Kitazawa H., Imamura M., Yokoyama T., Sakaguchi S., Nishida N., Mohri S., Aso H.
    • Organizer
      ASCB Annual Meeting. The American Society for Cell Biology
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Prion protein binding proteins of bovine intestine M cell.2012

    • Author(s)
      Nagasawa Y., Takahashi Y., Hondo T., Watanabe H., Terada S., Saito C., Someya S., Watanabe K., Ohwada S., Kitazawa H., Imamura M., Yokoyama T., Sakaguchi S., Nishida N., Mohri S., Aso H.
    • Organizer
      Asian Pacific Prion Symposium 2012
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 乳汁中リンパ球遊走因子シクロフィリンAの乳房炎早期診断マーカーへの応用2012

    • Author(s)
      麻生 久・堀越頼子・染谷俊輔・浦川めぐみ・板谷奈波・佐藤勝祥・佐藤秀俊・北澤春樹・松原朋子・菊 佳男・林 智人・渡邊康一
    • Organizer
      第17回日本乳房炎研究会学術集会
    • Place of Presentation
      東京、南青山会館
    • Related Report
      2012 Research-status Report
  • [Presentation] 高抗病性ランドレース豚の免疫担当臓器におけるサイトカイン発現の特性解析2012

    • Author(s)
      菅原静夏・染谷俊輔・渡邉一史・長澤裕哉・寺田俊介・住吉敬介・白須直樹・櫻田隆大・中野辰也・齋藤 遼・北澤春樹・島津朋之・鈴木啓一・大和田修一・渡邊康一・麻生 久
    • Organizer
      第62回東北畜産学会大会
    • Place of Presentation
      秋田、秋田県立大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 腸管M細胞における解糖系酵素アルドラーゼAを介した抗原取り込み機構2012

    • Author(s)
      長澤裕哉・高橋 遊・本堂哲也・渡邉一史・寺田俊介・染谷俊輔・渡邊康一・大和田修一・北澤春樹・今村守一・横山 隆・毛利資郎・麻生 久
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手、岩手大学
    • Related Report
      2012 Research-status Report
  • [Presentation] マウスDSS誘導大腸炎と白血球遊走因子シクロフィリンAとの関連性2012

    • Author(s)
      染谷俊輔・長澤裕哉・寺田俊介・渡邉一史・菅原静夏・板谷奈波・大和田修一・渡邊康一・麻生 久
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手、岩手大学
    • Related Report
      2012 Research-status Report
  • [Presentation] 乳房炎牛乳汁中に増量するシクロフィリンAの細胞遊走活性

    • Author(s)
      高梨 暁、他
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば市、国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 乳房炎多発症牛への枯草菌給与における血中樹状細胞の動態

    • Author(s)
      浦川めぐみ、他
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟市、新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 乳房炎発症牛への枯草菌給与における栄養因子への影響

    • Author(s)
      佐藤勝祥、他
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟市、新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 乳房炎発症牛への枯草菌給与における分娩後の栄養因子およびストレス指標の変動

    • Author(s)
      佐藤勝祥、他
    • Organizer
      第63回東北畜産学会大会
    • Place of Presentation
      山形市、山形テルサ
    • Related Report
      2013 Annual Research Report
  • [Presentation] 乳房炎多発症牛への枯草菌給与による乳房炎発症予防と血中樹状細胞の動態

    • Author(s)
      浦川めぐみ、他
    • Organizer
      第63回東北畜産学会大会
    • Place of Presentation
      山形市、山形テルサ
    • Related Report
      2013 Annual Research Report
  • [Book] 乳房炎の防除:乳質向上と免疫力アップ2012

    • Author(s)
      麻生久
    • Total Pages
      175
    • Publisher
      デーリー・ジャパン社
    • Related Report
      2013 Final Research Report
  • [Book] The Expression of Cytokeratins in Bovine Intestinal Microfold (M) Cells.(Cytokeratins-Tools in Oncology,Gerhard Hamilton (Ed.))2012

    • Author(s)
      Takashi Kanaya,Tetsuya Hondo,Kohtaro Miyazawa,Michael T. Rose and Hisashi Aso
    • Publisher
      InTech
    • Related Report
      2013 Final Research Report
  • [Book] 乳房炎の防除:乳質向上と免疫力アップ2012

    • Author(s)
      麻生 久
    • Publisher
      デーリー・ジャパン社
    • Related Report
      2012 Research-status Report
  • [Book] The Expression of Cytokeratins in Bovine Intestinal Microfold (M) Cells. In: Cytokeratins-Tools in Oncology, Gerhard Hamilton (Ed.)2012

    • Author(s)
      Takashi Kanaya, Tetsuya Hondo, Kohtaro Miyazawa, Michael T. Rose and Hisashi Aso
    • Publisher
      InTech
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/keitai/index-j.html

    • Related Report
      2013 Final Research Report
  • [Remarks] http://www.agri.tohoku.ac.jp/keitai/index-j.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 動物機能科学講座・機能形態学分野

    • URL

      http://www.agri.tohoku.ac.jp/keitai/index-j.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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