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A challenge to establish long-term primary culture of isolated muscle fibers

Research Project

Project/Area Number 24658228
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Zootechnical science/Grassland science
Research InstitutionKyushu University

Principal Investigator

IKEUCHI Yoshihide  九州大学, (連合)農学研究科(研究院), 教授 (90168112)

Co-Investigator(Kenkyū-buntansha) 辰巳 隆一  九州大学, 大学院農学研究院 (40250493)
水野 谷航 (水野谷 航)  九州大学, 大学院農学研究院 (20404056)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords筋線維タイプ / 骨格筋 / 筋線維 / ミオシン重鎖 / 細胞培養 / 筋萎縮 / 食肉 / 単離筋線維 / 運動 / 培養細胞
Outline of Final Research Achievements

To attain high quality meat production, elucidation of the regulation system of skeletal muscle fiber type is required. That mechanism will also contribute in medical and sports science. For detailed analysis of muscle fiber type, in the present study, we challenged to establish long term culture system of isolated rat muscle fibers (single fibers) and examined its characteristics. First, we succeeded in establishment of a culture protocol that can maintain more than 90% survival rate of muscle fibers isolated from rat up to 7 days. In the cultured isolated muscle fibers, fast type of myosin heavy chain isoform was significantly reduced over the culture period. We assumed this alteration can be similar to the state of muscle atrophy. Therefore, we measured protein level of atrogin-1, which is the muscle atrophy marker. The level of atrogin-1 was increased over time, suggesting the possibility that the present culture system can be applied as muscle atrophy in vitro model.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (41 results)

All 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (30 results) (of which Invited: 2 results) Remarks (2 results)

  • [Journal Article] Cold Exposure Increases Slow-Type Myosin Heavy Chain 1 (MyHC1) Composition of Soleus Muscle in Wistar Rats2014

    • Author(s)
      Wataru Mizunoya, Yohei Iwamoto, Yusuke Sato, Ryuichi A Tatsumi, Yoshihide Ikeuchi
    • Journal Title

      Animal Science Journal

      Volume: 85 Issue: 3 Pages: 293-304

    • DOI

      10.1111/asj.12143

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dietary fat influences the expression of contractile and metabolic genes in rat skeletal muscle2013

    • Author(s)
      Wataru Mizunoya, Yohei Iwamoto, Bungo Shirouchi, MASAO SATO, Yusuke Komiya, Farzaneh R. Razin, Ryuichi A Tatsumi, Yusuke Sato, Mako Nakamura, Yoshihide Ikeuchi
    • Journal Title

      PLOS ONE

      Volume: 8 Issue: 11 Pages: e80152-e80152

    • DOI

      10.1371/journal.pone.0080152

    • NAID

      120006536898

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preliminary Time-Course Study of Antiinflammatory Macrophage lnfiltration in Crush-Injured Skeletal Muscle.2013

    • Author(s)
      Shono J. Sakaguchi S. Suzuki T, et al.
    • Journal Title

      Animal Science Journal

      Volume: 84 Issue: 11 Pages: 744-750

    • DOI

      10.1111/asj.12105

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of 48-h Food Deprivation on the Expressions of Myosin Heavy-Chain Isoforms and Fiber Type-Related Factors in Rats2013

    • Author(s)
      Mizunoya, W., Sawano, S., Iwamoto, Y., Sato, Y., Tatsumi, R. and Ikeuchi, Y.
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 59 Issue: 4 Pages: 289-298

    • DOI

      10.3177/jnsv.59.289

    • NAID

      130004491351

    • ISSN
      0301-4800, 1881-7742
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Dietary Fat Type on Anxiety-Like and Depression-Like Behavior in Mice2013

    • Author(s)
      Wataru Mizunoya, Koichiro Ohnuki, Kento Baba, Hideo Miyahara, Naomi Shimizu, Kuniko Tabata, Takako Kino, Yusuke Sato, Ryuichi A Tatsumi, Yoshihide Ikeuchi
    • Journal Title

      SpringerPlus

      Volume: 2 Issue: 1 Pages: 165-165

    • DOI

      10.1186/2193-1801-2-165

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite ceels in vitro toward understanding its role in modulating2013

    • Author(s)
      Suzuki T et aj.
    • Journal Title

      ternational Journal of Biochemistry and Cell Biolo

      Volume: 45 Issue: 2 Pages: 476-482

    • DOI

      10.1016/j.biocel.2012.10.003

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Satellite ceels produce neural chemorepellent semaphorin 3A upon muscle injury.2013

    • Author(s)
      Sato Y, et al.
    • Journal Title

      Animal Science Journal

      Volume: 84 Issue: 2 Pages: 185-189

    • DOI

      10.1111/asj.12014

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle.2012

    • Author(s)
      DO MK, et al.
    • Journal Title

      Animal Science Journal

      Volume: 83 Issue: 10 Pages: 712-717

    • DOI

      10.1111/j.1740-0929.2012.01057.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Calcium infillx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch.2012

    • Author(s)
      Hara M, et al.
    • Journal Title

      American Journal of Physiology-Cell Physiology

      Volume: 302 Issue: 12 Pages: C1741-C1750

    • DOI

      10.1152/ajpcell.00068.2012

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 牛肉の筋線維タイプと遊離アミノ酸量の関係性2014

    • Author(s)
      岡 佳輝, 水野谷 航, 後藤 貴文, 友永 省三, 中村 真子, 辰巳 隆一, 池内 義秀
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヤマブシタケの摂取がマウス筋線維タイプ及び骨格筋の理化学的特性に及ぼす影響2014

    • Author(s)
      中村俊哉, 水野谷 航, 池内 義秀, 辰巳 隆一, 中村 真子
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Syndecan-2, 4 signal chemorepellent Sema3A expression in myogenic stem cells2014

    • Author(s)
      ド・マイコイ, 水野谷 航, 尾嶋孝一, 中村 真子, 辰巳 隆一, 池内 義秀
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋幹細胞による筋線維型自律制御機構は食品成分によって制御できる2014

    • Author(s)
      鈴木貴弘, 水野谷 航, 赤星 眞理子, 尾嶋孝一, 大坪秀明, 中村 真子, 池内 義秀, 和賀俊明, 辰巳 隆一
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋芽細胞の増殖・分化時におけるSema3AとPax7発現の相互作用に関する研究2014

    • Author(s)
      木 蘭, 中村 真子, 水野谷 航, 辰巳 隆一, 池内 義秀
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脱アミノ化酵素APOBEC2の欠損が筋再生に及ぼす影響2014

    • Author(s)
      大坪秀明, 佐藤祐介, 鈴木貴弘, 水野谷 航, 中村 真子, 辰巳 隆一, 池内 義秀
    • Organizer
      第118回日本畜産学会大会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Semaphorin 3A secreted from myogenic stem cells promotes slow-twitch muscle fiber generation2013

    • Author(s)
      Takahiro Suzuki, Koichi Ojima, Mai-Khoi Q. Do, Minako Hara, Wataru Mizunoya, Mako Nakamura, Yoshihide Ikeuchi, Judy E. Andeson, Ryuichi Tatsumi
    • Organizer
      2013 EMBO Workshop on“Semaphorin Function and Mechanism in Action”
    • Place of Presentation
      Cemay-la-Ville
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脱アミノ化酵素APOBEC2の欠損が筋細胞の分化に及ぼす影響2013

    • Author(s)
      大坪秀明, 佐藤祐介, 鈴木貴弘, 水野谷 航, 中村 真子, 辰巳 隆一, 池内 義秀
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] リンゴポリフェノールが筋衛星細胞の活性化に及ぼす影響2013

    • Author(s)
      安川沙也加, 水野谷 航, 中村 真子, 池内 義秀, 和賀俊明, 辰巳 隆一
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 牛乳由来ACE阻害ペプチドVPPがC2C12の筋分化に及ぼす影響2013

    • Author(s)
      木 蘭, 水野谷 航, 中村 真子, 辰巳 隆一, 池内 義秀
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 筋幹細胞由来の分泌因子Sema3Aによる筋線維型自律制御機構の解明2013

    • Author(s)
      鈴木貴弘, 尾嶋孝一, 大屋雄暉, 大坪秀明, 水野谷 航, 中村 真子, 池内 義秀, 辰巳 隆一
    • Organizer
      第117回日本畜産学会大会
    • Place of Presentation
      新潟大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] エイコサペンタエン酸はPPARδを介して筋線維タイプ関連遺伝子の発現に影響する:ラット単離筋線維を用いた検討2013

    • Author(s)
      水野谷 航, 小宮佑介, 後藤剛, 高橋信之, 河田照雄, 中村 真子, 佐藤祐介, Judy E. Anderson, 辰巳 隆一, 池内 義秀
    • Organizer
      第67回日本栄養・食糧学会大会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Eicosapentaenoic Acid Affects Skeletal Muscle Fiber Types relating factors via PPARδ Pathway in Isolated Fibers of Rats2013

    • Author(s)
      Wataru Mizunoya, Yusuke Komiya, Judy E. Anderson, Tsuyoshi Goto, Nobuyuki Takahashi, Teruo Kawada, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi
    • Organizer
      Keystone Symposia, Nuclear Receptors and Friends: Roles in Energy Homeostasis and Metabolic Dysfunction
    • Place of Presentation
      Alpbach Congress Centrum, Alpbach
    • Related Report
      2013 Annual Research Report
  • [Presentation] エイコサペンタエン酸はラット単離筋線維の脂肪代謝に関わる遺伝子発現を上昇させる2013

    • Author(s)
      小宮佑介ら
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] リンゴポリフェノールが培養筋管の筋線維タイプに及ぼす影響2013

    • Author(s)
      赤星眞理子ら
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] 筋線維の核内受容体PPARδを活性化する新規食品成分検索2013

    • Author(s)
      石井もも子ら
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] 活性化マクロファージは筋芽細胞の遊走性の調節に関与する2013

    • Author(s)
      坂口昇平ら
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] Syndecan-3 may implicate in regulation of neural chemorepellent Sema3A expression in satellite cells2013

    • Author(s)
      ド・マイコイら
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] 筋肉細胞分化時における筋幹細胞マーカーPax7とセマフォリン3Aの機能解明2013

    • Author(s)
      中村真子ら
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] 筋幹細胞による筋線維型の自律制御機構2013

    • Author(s)
      鈴木貴弘ら
    • Organizer
      第116回日本畜産学会大会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] 運動によるダイレクトな遺伝子発現制御の解明2013

    • Author(s)
      佐藤祐介
    • Organizer
      日本畜産学会若手企画委員会主催 懇親・交流会
    • Place of Presentation
      安田女子大学、広島市
    • Related Report
      2012 Research-status Report
  • [Presentation] Mechano-Sensing Calcium-Influx Machinery that Instigates Skeletal Muscle Satellite Cell Activation2012

    • Author(s)
      Tatsumi et al.
    • Organizer
      2012 FASEB Science Research Conference on “Skeletal Muscle Satellite & Stem Cells”
    • Place of Presentation
      Barga, Lucca, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] Semaphorin 3A regulates the early differentiation of satellite cells2012

    • Author(s)
      Suzuki et al.
    • Organizer
      2012 FASEB Science Research Conference on “Skeletal Muscle Satellite & Stem Cells”
    • Place of Presentation
      Barga, Lucca, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] Heparan/chondroitin sulfate chains may mediate HGF/FGF2-induced up-regulation of neural chemorepellent Sema3A in satellite cell cultures2012

    • Author(s)
      Do et al.
    • Organizer
      2012 FASEB Science Research Conference on “Skeletal Muscle Satellite & Stem Cells”
    • Place of Presentation
      Barga, Lucca, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] Change of skeletal muscle fiber types by n-3 poly unsaturated fatty acid2012

    • Author(s)
      Komiya et al.
    • Organizer
      2012 FASEB Science Research Conference on “Skeletal Muscle Satellite & Stem Cells”
    • Place of Presentation
      Barga, Lucca, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] APOBEC2 deficiency causes increased autophagy and abnormal mitochondria in skeletal muscle2012

    • Author(s)
      Sato et al.
    • Organizer
      2012 FASEB Science Research Conference on “Skeletal Muscle Satellite & Stem Cells”
    • Place of Presentation
      Barga, Lucca, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] 脂肪酸が骨格筋線維タイプに及ぼす影響2012

    • Author(s)
      水野谷航ら
    • Organizer
      精神・神経疾患研究開発費「筋ジストロフィーに対するトランスレーショナル・リサーチ」 の班会議(招待講演)
    • Place of Presentation
      JA共済ビル カンファエレンス・ホール, 東京都千代田区
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 神経軸索成長ガイダンス因子semaphorin 3Aによる筋線維型の制御2012

    • Author(s)
      鈴木貴弘ら
    • Organizer
      精神・神経疾患研究開発費「筋ジストロフィーに対するトランスレーショナル・リサーチ」 の班会議(招待講演)
    • Place of Presentation
      JA共済ビル カンファエレンス・ホール, 東京都千代田区
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] ペアードボックス転写因子Pax7とセマフォリン3Aによる筋肉細胞分化制御機構の解明2012

    • Author(s)
      中村真子ら
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡, 福岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] マウス骨格筋線維を用いた筋内脂肪量評価系の構築2012

    • Author(s)
      澤野祥子ら
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡, 福岡市
    • Related Report
      2012 Research-status Report
  • [Remarks] http://www.agr.kyushu-u.ac.jp/lab/muscle_and_meat/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 九州大学大学院農学研究院 資源生物科学部門 動物・海洋生物資源学講座 畜産化学分野のHP

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/muscle_and_meat/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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