Promotion of development of in vitro produced bovine embryos by using emulsified lipophilic nutrients

• Project/Area Number: 24658233
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: Kyoto University
• Principal Investigator: IKEDA Shuntaro 京都大学, (連合)農学研究科(研究院), 助教 (50447893)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ウシ / 受精卵 / 初期胚 / 体外受精 / 脂質 / 乳化 / 体外培養 / IVM-F-C / 胚盤胞 / 栄養と繁殖

Research Abstract

We developed a simple and stable method for emulsification of bioactive lipophilic nutrients intended to add them into culture media of in vitro produced bovine embryos. Emulsified mixture of alpha-tocopherol and beta-carotene (emVEBC) enhanced hatching rate of blastocysts, whereas emulsified oleic acid decreased blastocyst development. To elucidate mechanisms of the favorable effects of emVEBC on embryonic development, a microarray-based transcriptomic analysis of blastocysts was implemented and candidates of responsible genes were listed.

Research Products

• [Journal Article] Simple method for emulsification of lipophilic nutrients that affect preimplantation development of bovine embryos in vitro (2014)
  • Author(s): Ikeda S
  • Journal Title: Reprod. Fertil. Dev Volume: 26 Issue: 1 Pages: 157-157
  • DOI: 10.1071/rdv26n1ab86
  • Peer Reviewed
• [Journal Article] Astaxanthin ameliorates heat stress-induced impairment of blastocyst development in vitro : -Astaxanthin colocalization with and action on mitochondria- (2013)
  • Author(s): Kuroki T, Ikeda S, Okada T, Maoka T, Kitamura A, Sugimoto M, Kume S
  • Journal Title: J. Assist. Reprod. Genet Volume: 30 Issue: 5 Pages: 623-631
  • DOI: 10.1007/s10815-013-9987-z
  • Peer Reviewed
• [Presentation] Simple method for emulsification of lipophilic nutrients that affect preimplantation development of bovine embryos in vitro (2014)
  • Author(s): Ikeda S
  • Organizer: 40^<th> Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Reno, NV, USA
• [Presentation] Simple method for emulsification of lipophilic nutrients that affect preimplantation development of bovine embryos in vitro (2014)
  • Author(s): Ikeda S
  • Organizer: 40th Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Reno, NV, USA