| Project/Area Number |
24658242
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TSUJI Naotoshi 動物衛生研究所, 細菌・寄生虫研究領域, 主任研究員 (70355171)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 獣医学 / 感染症 / バベシア / ライブイメージング / 遺伝子改変原虫 |
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| Research Abstract |
The system for stable expression of green fluorescent protein (GFP) in babesia parasite was developed and the GFP-expressing parasite was observed through time-lapse video microscopy. (1) Stable expression of GFP in B. bovis using the WR99210/hDHFR selection system was achieved. The expression cassette was applied for the development of gene targeting (knockout) technique and the knockout parasite was established for the first time. (2) The gliding motility of B. bovis merozoites was revealed for the first time by in vitro time-lapse video microscopy of the GFP-expressing parasite. In vivo infection model of the parasite with SCID mice, with which a part of the circulating erythrocyte had been replaced with bovine cells, was also developed to investigate the gliding motility of the parasite in detail through time-lapse video microscopy.
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