Involvement of maternal mRNA in the regulation of differentiation during preimplantation development.

Research Project

Project/Area Number	24658244
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Basic veterinary science/Basic zootechnical science
Research Institution	The University of Tokyo
Principal Investigator	AOKI Fugaku 東京大学, 新領域創成科学研究科, 教授 (20175160)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	初期発生 / 細胞分化 / 母性mRNA / 2細胞期胚 / 4細胞期胚 / 初期胚 / 発生 / 分化 / 卵母細胞 / 卵
Research Abstract	To identify the maternal mRNA that are involved in the differentiation during preimplantation development, I conducted RNA sequencing analysis using the oocytes and embryos. The results showed that although 70% of maternal mRNAs were degraded soon after fertilization, the rest of them survived this wave of a rapid degradation. Especially, those from 691 genes were hardly degraded during the 1-cell stage. Gene Ontology (GO) enrichment analysis revealed that these genes were enriched in the GO terms categorized into transcription.