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Generation of transgenic mouse for analysis of in vivo dynamics of influenza virus infection

Research Project

Project/Area Number 24658245
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

OZAWA Makoto  鹿児島大学, 獣医学部, 准教授 (50568722)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsインフルエンザウイルス / トランスジェニックマウス / レポーター遺伝子 / 生体内感染動態
Research Abstract

To generate transgenic mouse ubiquitously expressing a recombinant influenza virus-like RNA encoding GFP gene, we constructed a plasmid for the expression of the recombinant RNA. Cultured cells pre-transfected with the constructed plasmid and subsequently infected with wild-type influenza viruses expressed GFP specifically in virus-infected cells. By injecting the transcriptional region fragment of the plasmid into mouse fertilized eggs, we obtained three mouse lines whose germlines were positive for the injected genes. The animals or their tail-derived primary cells, however, did not express GFP even after infection with wild-type influenza viruses. These results imply that the transcriptional region of the plasmid should be modified to complete this study.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (39 results)

All 2014 2013 2012 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 2 results) Presentation (23 results) (of which Invited: 1 results) Book (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] A recombinant influenza virus vaccine expressing the F protein of respiratory syncytial virus2014

    • Author(s)
      Fonseca W, Ozawa M, Hatta M, Orozco E, Martinez MB, Kawaoka Y
    • Journal Title

      Arch Virol

      Volume: 159 Pages: 1067-1077

    • DOI

      10.1007/978-1-61779-621-0_12

    • ISBN
      9781617796203, 9781617796210
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling2014

    • Author(s)
      Kasama Y, Mizukami T, Kusunoki H, Peveling-Oberhag J, Nishito Y, Ozawa M, Kohara M, Mizuochi T, Tsukiyama-Kohara K
    • Journal Title

      PLoS One

      Volume: 9(3) Issue: 3 Pages: 91373-91373

    • DOI

      10.1371/journal.pone.0091373

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent insights into hepatitis B virus-host interactions.2014

    • Author(s)
      Ezzikouri S, Ozawa M, Kohara M, Elmdaghri N, Benjelloun S, Tsukivama-Kohara K.
    • Journal Title

      Journal of Medical Virology

      Volume: 86 Issue: 6 Pages: 925-32

    • DOI

      10.1002/jmv.23916

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A recombinant influenza virus vaccine expressing the F protein of respiratory syncytial virus2014

    • Author(s)
      W. Fonseca, M. Ozawa, M. Hatta, E. Orozco, M. B. Martinez, Y. Kawaoka
    • Journal Title

      Arch Virol

      Volume: 159 Issue: 5 Pages: 1067-77

    • DOI

      10.1007/s00705-013-1932-z

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of H7N9 influenza A viruses isolated from humans.2013

    • Author(s)
      Watanabe T, Kiso M, Fukuyama S, Nakajima N, Imai M, Yamada S, Murakami S, Yamayoshi S, Iwatsuki-Horimoto K, Sakoda Y, Takashita E, McBride R, Noda T, Hatta M, Imai H, Zhao D, Kishida N, Shirakura M, de Vries RP, Shichinohe S, Okamatsu M, Tamura T, Tomita Y , Fujimoto N, Goto K, Katsura H, Kawakami E, Ishikawa I, Watanabe S, Ito M, Sakai-Tagawa Y, Sugita Y, Uraki R, Yamaji R, Eisfeld AJ, Zhong G, Fan S, Ping J, Maher EA, Hanson A, Uchida Y, Saito T, Ozawa M, Neumann G, Kida H, Odagiri T, Paulson JC, Hasegawa H, Tashiro M, Kawaoka Y
    • Journal Title

      Nature

      Volume: 501(7468) Issue: 7468 Pages: 551-5

    • DOI

      10.1038/nature12392

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel bivalent vaccine based on a PB2-knockout influenza virus protects mice from pandemic H1N1 and highly pathogenic H5N1 virus challenges.2013

    • Author(s)
      Uraki R, Kiso M, Iwatsuki-Horim oto K, Fukuyama S, Takashita E, Ozawa M, Kawaoka Y
    • Journal Title

      Journal of Virology

      Volume: 87(14) Issue: 14 Pages: 7874-81

    • DOI

      10.1128/jvi.00076-13

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic Effect of the PDZ and p85beta-Binding Domains of the NS1 Protein on Virulence of an Avian H5N1 Influenza A Virus2013

    • Author(s)
      Fan S, Macken CA, Li C, Ozawa M, Goto H, Iswahyudi NF, Nidom CA, Chen H, Neumann G, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 87 Issue: 9 Pages: 4861-4871

    • DOI

      10.1128/jvi.02608-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A cell-based high-throughput screening system for influenza A viral RNA transcription/replication inhibitors2013

    • Author(s)
      Ozawa, M, Shimojima M, Goto H, Watanabe S, Hatta Y, Kiso M, Furuta Y, Horimoto T, Peters NR, Hoffmann MF, Kawaoka Y
    • Journal Title

      Sci Rep

      Volume: 3 Issue: 1 Pages: 1106-1106

    • DOI

      10.1038/srep01106

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genomic polymorphisms in 3beta-hydroxysterol Delta24-reductase promoter sequences2013

    • Author(s)
      Salem NE, Saito M, Kasama Y, Ozawa M, Kawabata T, Harada S, Suda H, Asonuma K, El-Gohary A, Tsukiyama-Kohara K
    • Journal Title

      Microbiol Immunol

      Volume: 57 Pages: 179-184

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genomic polymorphisms in 3β-hydroxysterol Δ24-reductase promoter sequences.2013

    • Author(s)
      Salem NE, Saito M, Kasama Y, Ozawa M, Kawabata T, Harada S, Suda H, Asonuma K, El-Gohary A, Tsukiyama-Kohara K.
    • Journal Title

      Microbiolgy and Immunolgy

      Volume: 57 Issue: 3 Pages: 179-184

    • DOI

      10.1111/1348-0421.12025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intrinsic temperature sensitivity of Influenza C virus hemagglutinin-esterase-fusion protein2012

    • Author(s)
      E.Takashita
    • Journal Title

      J. Virol.

      Volume: 86 Issue: 23 Pages: 13108-13111

    • DOI

      10.1128/jvi.01925-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Competitive incorporation of homologous gene segments of influenza A virus into virions2012

    • Author(s)
      Inagaki A, Goto H, Kakugawa S, Ozawa M, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 86 Issue: 18 Pages: 10200-10202

    • DOI

      10.1128/jvi.01204-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Experimental adaptation of an influenza H5 haemagglutinin confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets2012

    • Author(s)
      M. Imai, T. Watanabe, M. Hatta, S. C. Das, M. Ozawa, K. Shinya, G. Zhong, A. Hanson, H. Katsura, S. Watanabe, C. Li, E. Kawakami, S. Yamada, M. Kiso, Y. Suzuki, E. A. Maher, G. Neumann, Y. Kawaoka
    • Journal Title

      Nature

      Volume: (電子版) Issue: 7403 Pages: 1-11

    • DOI

      10.1038/nature10831

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization in vitro and in vivo of pandemic (H1N1) 2009 influenza viruses isolated from patients2012

    • Author(s)
      Watanabe T, Imai M, Watanabe S, Shinya K, Hatta M, Li C, Neumann G, Ozawa M, Hanson A, Zhong G, Fukuyama S, Kawakami E, Simmons HA, Schenkman D, Brunner K, Capuano SV, 3rd, Weinfurter JT, Kilander A, Dudman SG, Suresh M, Hungnes O, Friedrich TC, Kawaoka Y
    • Journal Title

      J Virol

      Volume: 86 Issue: 17 Pages: 9361-9368

    • DOI

      10.1128/jvi.01214-12

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 豚インフルエンザの現状2013

    • Author(s)
      小澤真
    • Organizer
      鹿児島県養豚研究会平成24年度第2回研究会
    • Place of Presentation
      鹿児島
    • Year and Date
      2013-01-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ツル越冬地の水からのインフルエンザウイルスの分離2013

    • Author(s)
      小澤真、奥谷公亮、川畑淑子、永野希織、楠元勇、高瀬公三、小原恭子
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] Development and Aapplication of replication-incompetent foreign gene-expressing influenza viruses2013

    • Author(s)
      Makoto Ozawa and Yoshihiro Kawaoka
    • Organizer
      International Symposium of Korea Research Institute of Bioscience and Biotechnology
    • Place of Presentation
      Hwaseong (Republic of Korea)
    • Related Report
      2013 Final Research Report
  • [Presentation] 口蹄疫ウイルスのIRES依存性翻訳活性の評価2013

    • Author(s)
      神田雄大、小澤真、小原恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Final Research Report
  • [Presentation] ツパイとヒトとの遺伝的相同性の検証2013

    • Author(s)
      金澤伯弘、川畑淑子、永野希織、小原道法、小澤真、小原恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Final Research Report
  • [Presentation] ツルのねぐらにおけるインフルエンザウイルスの分離2013

    • Author(s)
      奥谷公亮、川畑淑子、永野希織、楠元勇、高瀬公三、小原恭子、小澤真
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Final Research Report
  • [Presentation] 養豚における抗インフルエンザウイルス抗体の保有状況2013

    • Author(s)
      小澤真、川畑淑子、出口栄三郎、小原恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Final Research Report
  • [Presentation] Influenza viral hemagglutinin-targeted macrocycles as an antiviral agent2013

    • Author(s)
      M. Saito, M. Ozawa, F. Yasui, T. Sasaki, T. Munakata, Y. Tobita, R. Ito, K. Munekata, K. Tsukiyama-Kohara, A. Sakurai, F. Shibasaki, Y. Sakoda, H. Kida, P. C. Reid, K. Kubota, H. Suga, Kohara
    • Organizer
      Options for the Control of Influenza VIII
    • Place of Presentation
      Cape Town (South Africa)
    • Related Report
      2013 Final Research Report
  • [Presentation] A cell-based screening system for influenza A viral RNA transcription/replication inhibitors2013

    • Author(s)
      M. Ozawa, M. Shimojima, H. Goto, S. Watanabe, Y. Hatta, M. Kiso, Y. Furuta, T. Horimoto, R. Peters, F. M. Hoffmann, Y. Kawaoka
    • Organizer
      15th International Negative Strand Virus Meeting
    • Place of Presentation
      Granada (Spain)
    • Related Report
      2013 Final Research Report
  • [Presentation] Development and Aapplication of replication-incompetent foreign gene-expressing influenza viruses2013

    • Author(s)
      Makoto Ozawa and Yoshihiro Kawaoka
    • Organizer
      International Symposium of Tokyo Metropolitan Institute of Medical Sccience
    • Place of Presentation
      Tokyo (Japan)
    • Related Report
      2013 Final Research Report
  • [Presentation] 生体内ライブイメージングを可能とする組換えA型インフルエンザウイルスの作出2012

    • Author(s)
      小澤真、福山聡、桂廣亮、石川いずみ、河岡義裕
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] 非増殖性・外来遺伝子発現組換えインフルエンザウイルスの作出とワクチン効果の検討2012

    • Author(s)
      小澤真、Sylvia Victor、渡辺真治、角川学士、桂廣亮、河岡義裕
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手
    • Related Report
      2013 Final Research Report
  • [Presentation] 豚におけるインフルエンザについて2012

    • Author(s)
      小澤真
    • Organizer
      南九州養豚会第111回定例会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Final Research Report
  • [Presentation] 非増殖性・外来遺伝子発現組換えインフルエンザウイルスの作出とワクチン効果の検討2012

    • Author(s)
      小澤真、Sylvia Victor、渡辺真治、角川学士、桂廣亮、河岡義裕
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手県盛岡市
    • Related Report
      2012 Research-status Report
  • [Presentation] 生体内ライブイメージングを可能とする組換えA型インフルエンザウイルスの作出2012

    • Author(s)
      小澤真、福山聡、桂廣亮、石川いずみ、河岡義裕
    • Organizer
      第60回日本ウイルス学会学術集会
    • Place of Presentation
      大阪府大阪市
    • Related Report
      2012 Research-status Report
  • [Presentation] A cell-based screening system for influenza A viral RNA transcription/replication inhibitors

    • Author(s)
      M. Ozawa, M. Shimojima, H. Goto, S. Watanabe, Y. Hatta, M. Kiso, Y. Furuta, T. Horimoto, N. R. Peters, F. M. Hoffmann, Y. Kawaoka
    • Organizer
      15th International Negative Strand Virus Meeting
    • Place of Presentation
      グラナダ(スペイン)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Influenza viral hemagglutinin-targeted macrocycles as an antiviral agent

    • Author(s)
      M. Saito, M. Ozawa, F. Yasui, T. Sasaki, T. Munakata, Y. Tobita, R. Ito, K. Munekata, K. Tsukiyama-Kohara, A. Sakurai, F. Shibasaki, Y. Sakoda, H. Kida, P. C. Reid, K. Kubota, H. Suga, M. Kohara
    • Organizer
      Options for the Control of Influenza VIII
    • Place of Presentation
      ケープタウン(南アフリカ共和国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 養豚における抗インフルエンザウイルス抗体の保有状況

    • Author(s)
      小澤 真、川畑 淑子、出口 栄三郎、小原 恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ツルのねぐらにおけるインフルエンザウイルスの分離

    • Author(s)
      奥谷 公亮、川畑 淑子、永野 希織、楠元 勇、高瀬 公三、小原 恭子、小澤 真
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ツパイとヒトとの遺伝的相同性の検証

    • Author(s)
      金澤 伯弘、川畑 淑子、永野 希織、小原 道法、小澤 真、小原 恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 口蹄疫ウイルスのIRES依存性翻訳活性の評価

    • Author(s)
      神田 雄大、小澤 真、小原 恭子
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学(岐阜)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ツル越冬地の水からのインフルエンザウイルスの分離

    • Author(s)
      小澤 真、奥谷 公亮、川畑 淑子、永野 希織、楠元 勇、高瀬 公三、小原 恭子
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development and application of replication-incompetent foreign gene-expressing influenza viruses

    • Author(s)
      Makoto Ozawa and Yoshihiro Kawaoka
    • Organizer
      3rd IGAKUKEN International Symposium on Control of Influenza and Hepatitis
    • Place of Presentation
      東京都世田谷区
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 専門家に聞くインフルエンザウイルス講座「インフルエンザウイルスの分類法―科・型・亜型―」インフルエンザ15(1)2014

    • Author(s)
      小澤真、河岡義裕
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] Influenza viruses with mutant PB2 gene segment as live attenuated vaccines2012

    • Inventor(s)
      Yoshihiro Kawaoka, Gabriele Neumann, Makoto Ozawa
    • Industrial Property Rights Holder
      Yoshihiro Kawaoka, Gabriele Neumann, Makoto Ozawa
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2012-08-24
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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