| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
The molecular mechanism of storage of avian hatching eggs is unclear. This study was focused on storage of chicken hatching eggs on developmental stage. Exposing chicken hatching eggs at all each stage of 35 to 15 degree, leading to a delay in hatching as compared to controls at 37.5 degree. This observation suggested that chicken hatching eggs could arrest embryonic development until stage of 35. Whole-transcriptome analysis of the embryonic heart incubated at 15 or 37.5 degrees showed that expression of 148 genes varied between arrest and resumption of embryonic development. Furthermore, in vitro and in vivo using quantitative PCR analysis of gene expression was associated with the cell cycle activation out of 148 genes indicated that one gene may be relevant to the mechanism of storage of avian hatching eggs.
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