| Project/Area Number |
24658255
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied veterinary science
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| Research Institution | Gifu University |
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Principal Investigator |
OHYA Kenji 岐阜大学, 応用生物科学部, 准教授 (50402219)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHI Hideto 岐阜大学, 応用生物科学部, 教授 (10156763)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 獣医学 / 病原細菌 / 偏性細胞内寄生細菌 / クラミジア / 細胞内増殖 / トランスクリプトーム / 人獣共通感染症 / RNAseq / 微生物 / 感染症 / ゲノム |
|---|
| Outline of Final Research Achievements |
Chlamydia, an obligate intracellular pathogen, possesses the peculiar developmental cycle involving biological conversion in host cells. In this study, aiming at revealing the regulatory mechanism of Chlamydial developmental cycle, transcriptional analysis (RNAseq) of Chlamydia in host cells was performed. There were few reports about RNA-seq analysis of obligate intracellular pathogens at that time. Prior to the RNA-seq analysis, we attempted enrichment of Chlamydial mRNA and depletion of host/Chlamydial rRNA in samples. Although novel transcribed regions including ncRNA were not identified, we obtained important information regarding about dealing with RNA of obligate intracellular pathogens toward RNA-seq. The RNA-seq of Chlamydia is ongoing in the continuous project.
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