Establishment of cell lines sensitive for virus isolation
| Project/Area Number |
24658257
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
MAEDA Ken 山口大学, 獣医学部, 教授 (90284273)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMOJIMA Masayuki 国立感染症研究所, ウイルス第一部, 室長 (10422411)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMODA Hiroshi 山口大学, 共同獣医学部, 助教 (40719887)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Fcレセプター / C型レクチン / 培養細胞 / DC-SIGN / DC-SIGNR / HUVEC / E型肝炎ウイルス / フェレット / アザラシ / クジラ / ウイルス |
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| Research Abstract |
1) We succeeded in establishment of cell lines expressing DC-SIGN and DC-SIGNR. These cell lines enhanced infection with Japanese encephalitis virus.
2) We developed cell lines expressing CD32 (Fc receptor) that can bind to antibody. This cell lines enhanced infection with feline infectious peritonitis virus under the antibody to the virus.
3)We succeeded in immortalization of human umbilical vein endothelial cells (HUVEC) by transfection with plasmid expressing SV40 Large T antige..
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Report
(3 results)
Research Products
(47 results)
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[Journal Article] Isolation of a novel herpesvirus from a Pacific white-sided dolphin2013
Author(s)
Noguchi, K., Shimoda, H., Terada, Y., Shimojima, M., Kohyama, K., Inoshima, Y., Maeda, K.
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Journal Title
Archives of Virology
Volume: 158
Issue: 3
Pages: 695-699
DOI
Related Report
Peer Reviewed
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[Journal Article] Feline infectious peritonitis virus with a large deletion in the 5'terminal region of spike gene retains its virulence for cats2012
Author(s)
Terada Y, Shiozaki Y, Shimoda H, Mahmoud HYA, Noguchi K, Nagao Y, Shimojima M, Iwata H, Mizuno T, Okuda M, Morimoto M, Hayashi T, Tanaka Y, Mochizuki M, Maeda K
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Journal Title
Journal of General Virology
Volume: 93
Pages: 1390-1934
Related Report
Peer Reviewed
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[Journal Article] Detection of bat coronaviruses from Miniopterus fuliginosus in Japan2012
Author(s)
Shirato K, Maeda K, Tsuda S, Suzuki K, Watanabe S, Shimoda H, Ueda N, Iha K, Taniguchi S, Kyuwa S, Endoh D, Matsuyama S, Kurane I, Saijo M, Morikawa S, Yoshikawa Y, Akashi H, Mizutani T
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Journal Title
Virus Genes
Volume: 44(1)
Pages: 40-44
Related Report
Peer Reviewed
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