Development of a rabies vaccine seed with high productivity and immunogenicity by modification of glycosylation
| Project/Area Number |
24658258
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 狂犬病 / ウイルス / ワクチン / N型糖鎖 / 免疫原性 / 狂犬病ウイルス |
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| Research Abstract |
In this work, we aimed to identify the rabies virus G protein applicative for an effective rabies vaccine by modification of the N-glycosylation. We found that the street virus G proteins with the multiple N-glycosylation additions did not enhance virus production compared with the G proteins with the single addition. The G protein with five additional N-glycosylation sites was able to produce few progeny viruses, but it recovered the function to produce progeny viruses in the presence of an inhibitor of cellular enzymes associated with N-glycosylation, indicating that this modified G protein may be applicative for development of a safe live vaccine strain with limited replication. Moreover, we found that the N-glycan addition, especially addition at position 194, enhanced the immunogenicity of the street virus G protein.
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Report
(3 results)
Research Products
(5 results)
