Basic study for development of a novel method for bovine mastitis

• Project/Area Number: 24658260
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied veterinary science
• Research Institution: Rakuno Gakuen University
• Principal Investigator: HIGUCHI HIDETOSHI 酪農学園大学, 獣医学群, 教授 (00305905)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 乳房炎 / 乳腺上皮細胞 / ＰＢＭＣ / 高度治療技術 / マイコプラズマ / ファージ / 治療技術 / ﾏｲｺﾌﾟﾗｽﾞﾏ

Outline of Final Research Achievements

Mycoplasma bovis is known as a significant pathogen and cause of large economic losses in beef and dairy calves worldwide. Numerous factors appear to play an important role in the development of disease during infection with M. bovis, e.g., inhibition of immune cell proliferation and induction of lymphocyte apoptosis. However, the mechanisms involved in M. bovis infections have not been explored and remain incompletely understood. We investigated the major cytokine mRNA expression of bovine mammary epithelial cells and bovine PBMC stimulated with M. bovis, and established mixed culture system for the evaluation of phage function. We clarified significant changes of cytokine gene expression of PBMC stimulated with M. bovis, and established mixed culture system consisted with mammary epithelial cells and PBMC. These results are important to develop novel methods to treat mycoplasma mastitis in dairy cows.

Research Products

• [Journal Article] paperCytokine mRNA profiling and the proliferative response ofbovine peripheral blood mononuclear cells to Mycoplasmabovis (2015)
  • Author(s): Satoshi Gondaira, Hidetoshi Higuchi, Hidetomo Iwano, Keiichi Nakajima,Kazuhiro Kawai, Shuhei Hashiguchi, Satoru Konnai, Hajime Nagahata
  • Journal Title: Vet. immune. Immunopathol. Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular Epidemiology of cases of Mycoplasma californicum infection in Japan. (2014)
  • Author(s): Hata, E., Suzuki K, Hanyu, H., Itoh, M., Higuchi, H., Kobayashi, H.
  • Journal Title: Appl. Environ Microbiol. Volume: 80 Issue: 24 Pages: 7717-7724
  • DOI: 10.1128/aem.02488-14
  • Peer Reviewed
• [Journal Article] 3.Antimicrobial Susceptibilities of Mycoplasma Isolated from Bovine Mastitis in Japan. (2014)
  • Author(s): Kawai, K., Higuchi, H., Iwano, H., Iwakuma, A., Onda, K., Sato, R., Hayashi, T., Nagahata, H. and Oshida
  • Journal Title: Anim. Sci. J. Volume: 86 Pages: 96-99
  • Peer Reviewed
• [Journal Article] Mycoplasma species isolated from intramammary infection of Japanese dairy cows. (2013)
  • Author(s): Higuchi, H., Gondaira, S., Iwano, H., Hirose, K., Nakajima, K., Kawai, K., Hagiwara, K., Tamura, Y., Nagahata, H.
  • Journal Title: Veterinary Record Volume: 172 Pages: 172-172
  • Peer Reviewed
• [Journal Article] Effects of vitamin E supplementation on cellular α-tocopherol concentrations of neutrophils in Holstein calves. (2013)
  • Author(s): Higuchi H, Ito E, Iwano H, Oikawa S, Nagahata H.
  • Journal Title: Canadian Journal of Veterinary Research Volume: 77 Pages: 120-125
  • Peer Reviewed
• [Journal Article] Mycoplasma species isolated from intramammary infection of Japanese dairy cows. (2013)
  • Author(s): Higuchi H, Gondaira S, Iwano H, Hirose K, Nakajima K, Kawai K, Hagiwara K, Tamura Y, Nagahata H
  • Journal Title: Veterinary Record Volume: In press
  • Peer Reviewed
• [Journal Article] Mycoplasma infection in the uterus of early postpartum dairy cows and its relation to dystocia and endometritis. (2012)
  • Author(s): Ghanem, M. E., Higuchi, H., Tezuka, E., Ito, H., Devkota, B., Izaike, Y. and Osawa, T
  • Journal Title: Theriogenology Volume: 79 Pages: 180-185
  • Peer Reviewed
• [Presentation] 子牛鼻腔内マイコプラズマ種の浸潤状況 (2014)
  • Author(s): 吉田慎吾・樋口豪紀・権平 智・岩野英知・河合一洋・佐藤豊孝・
  • Organizer: 日本マイコプラズマ学会
  • Place of Presentation: 東京大学
  • Year and Date: 2014-05-22 – 2014-05-23
• [Presentation] 日本の酪農場におけるマイコプラズマ乳房炎の浸潤状況 (2014)
  • Author(s): 権平智・樋口豪紀・岩野英知・河合一洋・田村豊・永幡肇
  • Organizer: 日本マイコプラズマ学会
  • Place of Presentation: 東京大学
  • Year and Date: 2014-05-22 – 2014-05-23
• [Presentation] 日本の酪農場におけるマイコプラズマ種の浸潤状況
  • Author(s): 樋口豪紀・岩野英知
  • Organizer: 日本細菌学会
  • Place of Presentation: 東京