| Project/Area Number |
24658261
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
MAEDA Akihiko 京都産業大学, 総合生命科学部, 教授 (70333359)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Nobuya 北里大学, 獣医学部, 教授 (20302614)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 感染症 / フラビウイルス / スライス培養 / 臓器特異性 / ウイルス / 獣医学 / 細胞・組織 / 臓器 / ウエストナイルウイルス / ウイルス様粒子 |
|---|
| Research Abstract |
In order to solidify organ-tropisms of pathogenic microbes, we conducted the experiments as described below, using a neurotropic flavivirus as a model of infectious agent. First, flavivirus reporter virus particles (RVPs) encoding the red-fluorescent protein gene within its genome RNA, were prepared to be visible virus infections. Next, mouse brain slice-culture system was established to analyze organ-tropism of the virus easily. Finally, flavivirus RVPs-infection system on mouse brain slice-cultures was established. We might apply the system to do research on organ-tropism of pathogenic microbes.
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