Genomic analysis of histiocytic proliferative disorders in Pembroke Welsh corgi dog

• Project/Area Number: 24658263
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: UCHIDA Kazuyuki 東京大学, 農学生命科学研究科, 准教授 (10223554)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 組織球性肉腫 / イヌ / 遺伝子異常 / コーギー / コーギー犬 / 遺伝性疾患 / マクロファージ / 樹状細胞 / 病因遺伝子

Research Abstract

The aim of this research project is to clarify the molecular pathogenesis of the histiocytic sarcomas in Pembroke Welsh corgi dogs (PWC). To know the prevalence of histiocytic sarcomas of the PWC in Japan, an epidemiological study for histiocytic sarcomas of dogs in Veterinary Medical Center of the University of Tokyo, was conducted. The results revealed the high incidence of histiocytic sarcomas in the PWC, unlike the previous reports from foreign countries. We also analyzed the DNA of the affected PWC using next-generation sequencers, but so far yet to identify the gene mutations directly associated to tumoregenesis of histiocytic sarcomas. Besides, the cell line from histiocytic sarcoma from a PWC dog has been successfully established, that would be the useful experimental tools for the investigation of the tumoregenesis of histiocytic sarcoma in PWC dogs.

Research Products

• [Journal Article] Clinical Characteristics and Prognostic Factors in Dogs with Histiocytic Sarcomas in Japan (2014)
  • Author(s): Takahashi M1, Tomiyasu H, Hotta E, Asada H, Fukushima K, Kanemoto H, Fujino Y, Ohno K, Uchida K, Nakayama H, Tsujimoto H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 5 Pages: 661-666
  • DOI: 10.1292/jvms.13-0414
  • NAID: 130003391271
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Presentation] イヌ組織球性肉腫細胞におけるEMR4の機能解析およびシグナル探索 (2013)
  • Author(s): 坪井誠也,井手鉄哉,渡邊学,内田和幸,菅野純夫,中山裕之
  • Organizer: 2013獣医内科学アカデミー
  • Place of Presentation: パシフィコ横浜
• [Presentation] イヌ組織球性肉腫細胞におけるEMR4 の機能解析およびシグナル探索 (2013)
  • Author(s): 坪井誠也（他5名）
  • Organizer: 日本獣医内科学アカデミー
  • Place of Presentation: 横浜市
• [Remarks]
  • URL: http://www.vm.a.u-tokyo.ac.jp/byouri/research03/research03.htm