Is "steric hindrance effect" the possible new concept for tumor metastasis mechanism?

• Project/Area Number: 24658268
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Yamaguchi University
• Principal Investigator: MIZUNO Takuya 山口大学, 獣医学部, 教授 (90398826)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Takayuki 東京大学, 農学部, 助教 (40447363) ; KUBO Masato 山口大学, 共同獣医学部, 助教 (40593439)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: PSGL-1 / 犬 / 腫瘍

Research Abstract

The effect of PSGL-1 expression on tumor cells on tumor metastasis was examined. Canine PSGL-1 was not detected in naturally occurred canine solid tumor by immunohistochemistry, but was detected in canine lymphoma samples. Injection of canine lymphoma cell lines which has lower level of PSGL1 by knockdown technique into NOJ mice had lower rate of metastasis as compared with parental lymphoma cell lines. Canine mammary gland tumor cell lines overexrpressing PSGL-1 into NOD/SCID mice appeared to have more lung metastasis. In conclusion, this study indicated that PSGL-1 has an important role in tumor metastasis or dissemination.

Research Products

• [Journal Article] Seroepidemiology of Reovirus in Healthy Dogs in Six Prefectures in Japan (2014)
  • Author(s): Hwang CC, Mochizuki M, Maeda K, Okuda M, Mizuno T
  • Journal Title: J Vet Med Sci Volume: 76(3) Pages: 471-475
  • NAID: 130003382393
  • Peer Reviewed
• [Journal Article] Reovirus type-2-triggered autoimmune cholangitis in extrahepatic bile ducts of weanling DBA/1J mice (2014)
  • Author(s): Nakashima T, Hayashi T, Tomoeda S, Yoshino M, Mizuno T
  • Journal Title: Pediatr Res Volume: 75(1-1) Pages: 29-37
  • Peer Reviewed
• [Journal Article] Oncolytic reovirus in canine mast cell tumor (2013)
  • Author(s): Hwang CC, Umeki S, Kubo M, Hayashi T, Shimoda H, Mochizuki M, Maeda K, Baba K, Hiraoka H, Coffey M, Okuda M, Mizuno T
  • Journal Title: PLoS ONE Volume: 8(9)
  • Peer Reviewed
• [Journal Article] Anti-adhesive property of P-selectin glycoprotein ligand-1 (PSGL-1) due to steric hindrance effect (2013)
  • Author(s): Umeki S, Suzuki R, Ema Y, Shimojima M, Nishimura Y, Okuda M, Mizuno T
  • Journal Title: J Cell Biochem Volume: 114(6) Pages: 1271-1285
  • Peer Reviewed
• [Journal Article] A Potential Therapeutic Application of SET/I2PP2A Inhibitor OP449 for Canine T-Cell Lymphoma (2013)
  • Author(s): Fujiwara N, Kawasaki H, Yabe R, Christensen DJ, Vitek MP, Mizuno T, Sato K, Ohama T
  • Journal Title: J Vet Med Sci Volume: 75(3) Pages: 349-354
  • NAID: 130001879628
  • Peer Reviewed
• [Journal Article] Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells (2013)
  • Author(s): Shimizu K, Mizuno T, Shinga J, Asakura M, Kakimi K, Ishii Y, Masuda K, Maeda T, Sugahara H, Sato Y, Matsushita H, Nishida K, Hanada KI, Dörrie J, Schaft N, Bickham K, Koike H, Ando T, Nagai R, Fujii SI
  • Journal Title: Cancer Res Volume: 73(1) Pages: 62-73
  • Peer Reviewed
• [Journal Article] Anti-adhesive property of P-selectin glycoprotein ligand-1 (PSGL-1)due to steric hindrance effect. (2013)
  • Author(s): Umeki S, Suzuki R, Ema Y, Shimojima N, Nishinura Y, Okuda M, Mizuno T.
  • Journal Title: J Cell Biochem. Volume: 114 (6) Issue: 6 Pages: 1271-1285
  • DOI: 10.1002/jcb.24468
  • Peer Reviewed
• [Journal Article] Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model. (2013)
  • Author(s): Umeki S, Ema Y, Suzuki R, Kubo M, Hayashi T, Okamura Y, Yamazaki J, Tsujimoto H, Tani K, Hiraoka H, Okuda M, Mizuno T.
  • Journal Title: J Vet Med Sci. Volume: -
  • NAID: 130002502194
  • Peer Reviewed
• [Journal Article] Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model (2012)
  • Author(s): Umeki S, Ema Y, Suzuki R, Kubo M, Hayashi T, Okamura Y, Yamazaki J, Tsujimoto H, Tani K, Hiraoka H, Okuda M, Mizuno T
  • Journal Title: J Vet Med Sci
  • NAID: 130002502194
  • Peer Reviewed
• [Journal Article] Administration of interferon (IFN)-αexacerbates Reovirus type-2-triggered autoimmune insulitis in DBA/1J mice (2012)
  • Author(s): Nakashima T, Hayashi T, Yamamoto Y, Mizuno T
  • Journal Title: Scand J Immunol
  • Peer Reviewed
• [Journal Article] Feline infectious peritonitis virus with a large deletion in the 5'-terminal region of the spike gene retains its virulence for cats (2012)
  • Author(s): Terada Y, Shiozaki Y, Shimoda H, Mahmoud HY, Noguchi K, Nagao Y, Shimojima M, Iwata H, Mizuno T, Okuda M, Morimoto M, Hayashi T, Tanaka Y, Mochizuki M, Maeda K
  • Journal Title: J Gen Virol Volume: 93(Pt 9) Pages: 1930-934
  • Peer Reviewed
• [Journal Article] Reovirus type-2 infection in newborn DBA/1J mice reduces the development of late allergic asthma (2012)
  • Author(s): Nakashima T, Hayashi T, Mizuno T
  • Journal Title: Int J Exp Patho Volume: 93(3) Pages: 234-242
  • Peer Reviewed
• [Journal Article] Ehrlichia canis Infection in Two Dogs that Emigrated from Endemic Areas (2012)
  • Author(s): Baba K, Itamoto K, Amimoto A, Kitagawa K, Hiraoka H, Mizuno T, Sato H, Okuda M
  • Journal Title: J Vet Med Sci Volume: 74(6) Pages: 775-778
  • NAID: 130002090067
  • Peer Reviewed
• [Journal Article] Development of High-Grade B-Cell Lymphoma Concurrent with T-Cell Chronic Lymphocytic Leukemia in a Dog (2012)
  • Author(s): Okawa T, Hiraoka H, Wada Y, Baba K, Itamoto K, Mizuno T, Okuda M
  • Journal Title: J Vet Med Sci Volume: 74(5) Pages: 677-80
  • NAID: 130001879878
  • Peer Reviewed
• [Journal Article] Destruction of salivary and lacrimal glands by Th1-polarized reaction in a model of secondary Sjogren's syndrome in lupus-prone female NZB x NZWF(1) mice (2012)
  • Author(s): Hayashi T, Shimoyama N, Mizuno T
  • Journal Title: Inflammation Volume: 35 (2) Pages: 638-646
  • Peer Reviewed
• [Journal Article] Calreticulin expression in neoplastic versus normal dog mammary glands : A cDNA subtraction-based study (2012)
  • Author(s): Okawa T, Kurio Y, Morimoto M, Hayashi T, Nakagawa T, Sasaki N, Okuda M, Mizuno T
  • Journal Title: Res. Vet. Sci Volume: 92(1) Pages: 80-91
  • Peer Reviewed
• [Presentation] 日本国内の主要犬種における犬白血球抗原I型の遺伝子解析 (2013)
  • Author(s): 中谷亮太、井上公美、川野浩志、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-09-21
• [Presentation] レオウイルスによる犬の各種腫瘍細胞株に対する細胞死の誘導 (2013)
  • Author(s): 伊賀瀬雅也、Chung Chew Hwang、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-09-21
• [Presentation] Reovirus effectively induces oncolysis in canine mast cell tumor in vivo and ex vivo Chung Chew Hwang (2013)
  • Author(s): 梅城沙織、伊賀瀬雅也、久保正仁、林俊春、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-09-21
• [Presentation] イヌインターフェロンガンマによる犬腫瘍細胞株の増殖抑制効果の検討 (2013)
  • Author(s): 濱村友紀、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-09-21
• [Presentation] イヌのリンパ腫において過剰発現する遺伝子IL-37の解析 (2013)
  • Author(s): 片山貴朗、柳瀬拓磨、藤原亜紀、辻本元、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学(岐阜市)
  • Year and Date: 2013-09-21
• [Presentation] 人工アジュバントベクター細胞を用いた犬の腫瘍免疫療法の前臨床試験 (2013)
  • Author(s): 水野拓也、清水佳奈子、垣見和宏、藤井眞一郎
  • Organizer: 獣医アトピー・アレルギー・免疫学会第7回シンポジウム症例報告
  • Place of Presentation: 国際ファッションセンター3F KFCホールアネックス(東京都))
  • Year and Date: 2013-01-13
• [Presentation] イヌのリンパ腫において過剰発現する遺伝子の解析 (2012)
  • Author(s): 柳瀬拓磨、梅城沙織、酒井治、平岡博子、藤原亜紀、辻本元、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市
  • Year and Date: 2012-09-15
• [Presentation] イヌのWT-1に対する抗体の特異性の解析 (2012)
  • Author(s): 酒井治、中谷亮太、梅城沙織、酒井洋樹、久保正仁、森本将弘、林俊春、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市
  • Year and Date: 2012-09-15
• [Presentation] 新しく樹立したイヌリンパ腫細胞株のin vitroおよびin vivoにおける性状解析 (2012)
  • Author(s): 梅城沙織、江馬康夫、鈴木綾一、久保正仁,林俊春、岡村泰彦、山崎淳平、辻本元、谷健二、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市
  • Year and Date: 2012-09-15
• [Presentation] Discovering the oncolytic potential of reovirus in veterinary medicine : reovirus-induced cell death in canine lymphoma and mast cell tumor cell lines (2012)
  • Author(s): Hwang Chung Chew、梅城沙織、中川貴之、下田宙、望月雅美、前田健、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市
  • Year and Date: 2012-09-15
• [Presentation] イヌのリンパ腫において過剰発現する遺伝子の解析 (2012)
  • Author(s): 柳瀬拓磨、梅城沙織、酒井治、平岡博子、藤原亜紀、辻本元、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学（盛岡市）
• [Presentation] イヌのWT-1に対する抗体の特異性の解析 (2012)
  • Author(s): 酒井治、中谷亮太、梅城沙織、酒井洋樹、久保正仁、森本将弘、林俊春、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学（盛岡市）
• [Presentation] 新しく樹立したイヌリンパ腫細胞株のin vitroおよびin vivoにおける性状解析 (2012)
  • Author(s): 梅城沙織、江馬康夫、鈴木綾一、久保正仁，林俊春、岡村泰彦、山崎淳平、辻本元、谷健二、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学（盛岡市）
• [Presentation] Discovering the oncolytic potential of reovirus in veterinary medicine : reovirus-induced cell death in canine lymphoma and mast cell tumor cell lines (2012)
  • Author(s): Hwang Chung Chew、梅城沙織、中川貴之、下田宙、望月雅美、前田健、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学（盛岡市）
• [Remarks]
  • URL: http://web.cc.yamaguchi-u.ac.jp/~vintmed/mizuno/