Generation of hematopoietic cells from canine induced pluripotent stem cells for cell transplantation

• Project/Area Number: 24658270
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: INABA TOSHIO 大阪府立大学, 生命環境科学研究科(系), 教授 (00137241)
• Co-Investigator(Kenkyū-buntansha): SHUGIURA Kikuya 大阪府立大学, 生命環境科学研究科, 准教 (30171143) ; HATOYA Shingo 大阪府立大学, 生命環境科学研究科, 助教 (40453138)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 再生医療 / 統合動物科学 / iPS細胞 / イヌ / サイトカイン / バイオテクノロジー / トランスレーショナルリサーチ

Research Abstract

The objective of this study was in the development of the effective method for generation of hematopoietic cells from canine induced pluripotent stem (iPS) cells for cell transplantation, and the following results were obtained. We successfully cloned three canine hematopoietic cytokine genes and produced their proteins. We reprogrammed adult dog skin fibroblasts into iPS-like cells, using 4 transcription factors. While the cell colonies showed negative alkaline phosphatase activity, they were maintained until 24 passages. Erythroblasts could be induced from feline iPS cells, using several hematopoietic cytokines. This method will apply to canine iPS cells.

Research Products

• [Journal Article] Evaluation of serum phosphotylated neurofilament subunit NF-H as a prognostic biomarker in dogs with thoracolumbar intervertebral disc herniation. (2014)
  • Author(s): Nishida H, Nakayama M, Tanaka H, Kamishima H, Izawa T, Hatoya S, Sugiura K, Ide C, Inaba T
  • Journal Title: Vet. Surg. Volume: 43 Issue: 3 Pages: 289-293
  • DOI: 10.1111/j.1532-950x.2014.12144.x
  • Peer Reviewed
• [Journal Article] Clinical Study Report on Milk Production in the Offspring of a Somatic Cell Cloned Holstein Cow (2013)
  • Author(s): Takahashi, M., Sawada, K., Kawate, N., Inaba, T., Tamada, H.
  • Journal Title: Journal of Reproduction and Development Volume: 59 Issue: 6 Pages: 595-598
  • DOI: 10.1262/jrd.2013-025
  • NAID: 130003361057
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy. (2013)
  • Author(s): Wijewardana, V., Sugiura, K., Yahata, M., Akazawa, T., Wijesekera, D. P., Imamoto, S., Hatoya, S., Inoue, N., Inaba, T.
  • Journal Title: Vet. Immunol. Immunopathol. Volume: 156 Issue: 1-2 Pages: 121-127
  • DOI: 10.1016/j.vetimm.2013.09.016
  • Peer Reviewed
• [Journal Article] Generation of functional platelets from canine induced pluripotent stem cells. (2013)
  • Author(s): Nishimura, T., Hatoya, S., Kanegi, R., Sugiura, K., Wijewardana, V., Kuwamura, M., Tanaka, M., Yamate, J., Izawa, T., Takahashi, M., Kawate, N., Tamada, H., Imai, H., Inaba, T.
  • Journal Title: Stem Cells Dev. Volume: 22 Issue: 14 Pages: 2026-2035
  • DOI: 10.1089/scd.2012.0701
  • Peer Reviewed
• [Journal Article] Improvement of Superovulatory Response and Pregnancy Rate after Transfer of Embryos Recovered from Japanese Black Cows Fed Rumen Bypass Polyunsaturated Fatty Acids (2013)
  • Author(s): Takahashi, M., Sawada, K., Kawate, N., Inaba, T., Tamada, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 11 Pages: 1485-1490
  • DOI: 10.1292/jvms.12-0235
  • NAID: 130003362257
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Presentation] Improvement of dendritic cell-based immunotherapy for enhancing systemic immune responses against cancer (2013)
  • Author(s): Wijewardana, V., Sugiura, K., Akazawa, T., Wijesekera, D.-P.H., Imamoto, S., Inoue, N., Inaba, T.
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2013-12-12
• [Presentation] イヌiPS細胞から神経系細胞への分化誘導培養系に及ぼす骨髄間質細胞の影響 (2013)
  • Author(s): 因野由衣子、西村俊哉、西田英高、鳩谷晋吾、杉浦喜久弥、川手憲俊、高橋正弘、玉田尋通、稲葉俊夫
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
  • Year and Date: 2013-09-20
• [Presentation] イエネコ体細胞を用いた人工多能性幹細胞の誘導 (2013)
  • Author(s): 古田侑吾、川口高正、南直治郎、山田雅保、稲葉俊夫、今井裕
  • Organizer: 第63回関西畜産学会
  • Place of Presentation: 滋賀
  • Year and Date: 2013-09-05
• [Presentation] Generation of functional platelets from canine induced pluripotent stem cells (2013)
  • Author(s): Nishimura, T., Hatoya, S., Kanegi, R., Sugiura, K., Wijewardana, V., Kuwamura, M., Tanaka, M., Takahashi, M., Kawate, N., Tamada, H., Imai, H., Inaba, T.
  • Organizer: The International Society for Stem Cell Research (ISSCR) 11th Annual Meeting
  • Place of Presentation: 米国ボストン
  • Year and Date: 2013-06-15
• [Presentation] Generation of induced pluripotent stem cells and production of leukemia inhibitory factor (LIF) in domestic cats (2013)
  • Author(s): Kanegi, R., Hatoya, S., Takenaka, S., Nishimura, T., Wijewardana, V., Sugiura, K., Takahashi, M., Kawate, N., Tamada, H., Inaba, T.
  • Organizer: The International Society for Stem Cell Research (ISSCR) 11th Annual Meeting
  • Place of Presentation: 米国ボストン
  • Year and Date: 2013-06-14
• [Presentation] Generation of induced pluripotent stem cells and production of leukemia inhibitory factor (LIF) in domestic cats. (2013)
  • Author(s): Kanegi, R., Hatoya, S., Takenaka, S., Nishimura, T., Wijewardana, V., Sugiura, K., Takahashi, M., Kawate, N., Tamada, H., Inaba, T.
  • Organizer: The International Society for Stem Cell Research (ISSCR) 11th Annual Meeting
  • Place of Presentation: 米国ボストン
• [Presentation] イヌiPS細胞から神経系細胞への分化誘導 (2013)
  • Author(s): 因野由衣子、西村俊哉、西田英高、鳩谷晋吾、杉浦喜久弥、川手憲俊、高橋正弘、玉田尋通、稲葉俊夫
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
• [Presentation] ネコiPS細胞株樹立と多能性維持タンパク質ネコLIFの作製 (2012)
  • Author(s): 金城綾二、鳩谷晋吾、杉浦喜久弥、竹中重雄、玉田尋通、川手憲俊、高橋正弘、稲葉俊夫
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手
  • Year and Date: 2012-09-14
• [Presentation] イヌiPS細胞の作製と血小板への分化誘導 (2012)
  • Author(s): 西村俊哉、鳩谷晋吾、杉浦喜久弥、今井裕、玉田尋通、川手憲俊、高橋正弘、稲葉俊夫
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手
  • Year and Date: 2012-09-14
• [Remarks]
  • URL: http://kyoindb.acs.osakafu-u.ac.jp/profile/out.cudjDYHfHMO.PEVGDxV-iw==.html
• [Remarks] Laboratory of Cell Pathobiology
  • URL: http://www.vet.osakafu-u.ac.jp/cellE/cellE.html
• [Remarks] Laboratory of Cell Pathobiology
  • URL: http://www.vet.osakafu-u.ac.jp/cellE/cellE.html
• [Patent(Industrial Property Rights)] イヌのｉＰＳ細胞の作製方法 (2013)
  • Inventor(s): 稲葉俊夫、西村俊哉ら
  • Industrial Property Rights Holder: 公立大学法人大阪府立大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-033588
  • Filing Date: 2013-02-22