| Project/Area Number |
24658275
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Boundary agriculture
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMOTO Jun 京都大学, 化学研究所, 助教 (90511238)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 酵素 / 応用微生物 / 有機ハロゲン化合物 / デハロゲナーゼ / フルオロ酢酸 |
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| Outline of Final Research Achievements |
Structures and catalytic mechanisms of microbial enzymes that catalyze the degradation of persistent organohalogen compounds were studied. As to fluoroacetate dehalogenase, it was suggested that interaction of the fluorine atom of the substrate with His149, Trp 150, and Tyr212 is important for the defluorination reaction. As to DL-2-haloacid dehalogenase, two substrate-binding sites were found, and it was suggested that the dehalogenation reaction proceeds in one of these sites. As to 2-haloacrylate hydratase, we established a protein purification method and searched for crystallization condition for X-ray crystallographic analysis of the enzyme structure.
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