Development of immune-response free nano-particles using fungal biosurfactant protein
| Project/Area Number |
24658281
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
ABE Keietsu 東北大学, (連合)農学研究科(研究院), 教授 (50312624)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAMI Seiichi 東北大学, 多元物質科学研究所, 准教授 (40311550)
FUKUMOTO Manabu 東北大学, 加齢医学研究所, 教授 (60156809)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAKAMI Kazuyoshi 東北大学, 医学(系)研究科, 教授 (10253973)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | hydrophobin / ナノ粒子 / 免疫応答 / マクロファージ / 貪食 / イメージング / 糸状菌 / Aspergillus / バイオセンサー / 医療用ナノ粒子 / 界面活性蛋白質 / 免疫応答回避 |
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| Research Abstract |
Although nano-particles have been developed for medical treatments, immune-responses including phagocytosis by macrophages are a major obstacle for medical use. Recently, a biosurfactant protein, hydrophobin originating from conidia of the pathogenic fungus Aspergillus fumigatus has been shown as an escaping factor from host immune-systems (stealth factor). In the present study, we developed novel stealth nano particles coated with the hydrophobin RolA of the industrial fungus Aspergillus oryzae. Fe3O4 nano-particles were coated with purified RolA. When RolA-coated particles were incubated with dendritic cells, cytokine production from dendritic cells was not stimulated. RolA-coated particles showed lower levels of phagocytosis by macrophages than non-coated particles. When RolA-coated particles were administered to mice by intravenous injection, RolA-coated particles indicated significantly lower levels of accumulation to the liver and spleen than non-coated particles by MRI-imaging.
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Report
(3 results)
Research Products
(25 results)
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[Presentation] 糸状菌由来の免疫回避機能性素材を用いた新規医療用ナノ粒子の開発2013
Author(s)
佐藤大貴,松村香菜,高橋慎太郎,高橋徹,村垣公英,石井恵子,川上和義,冨樫貴成,高見誠一,阿尻雅文,福本学,阿部敬悦
Organizer
第13回糸状菌分子生物学コンファレンス
Place of Presentation
筑波(ポスター賞)
Related Report
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[Presentation] 糸状菌由来の免疫回避機能性素材を用いた新規医療用ナノ粒子の開発ナノ粒子の性質について2013
Author(s)
佐藤大貴,松村香菜,高橋慎太郎,高橋徹,村垣公英,石井恵子,川上和義,冨樫貴成,高見誠一,阿尻雅文,福本学,阿部敬悦
Organizer
日本農芸化学会2013年大会
Place of Presentation
仙台(トピックス賞)
Related Report
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[Presentation] 糸状菌由来の免疫回避機能性素材を用いた新規医療用ナノ粒子の開発2013
Author(s)
佐藤大貴, 松村香菜, 高橋慎太郎, 高橋徹, 村垣公英, 石井恵子, 川上和義, 冨樫貴成, 高見誠一, 阿尻雅文, 福本学, 阿部敬悦
Organizer
第13回糸状菌分子生物学コンファレンス
Place of Presentation
つくば国際会議場, 文部科学省研究交流センター (つくば市)
Related Report
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