| Project/Area Number |
24658286
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
MARUYAMA NOBUYUKI 京都大学, (連合)農学研究科(研究院), 准教授 (90303908)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 種子 / 貯蔵タンパク質 / 液胞 / 選別輸送シグナル / レセプター / 細胞内輸送 / 受容体 / 高次構造 |
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| Research Abstract |
Plant vacuoles have many functions including a reservation of seed storage proteins. It is suggested that two types of vacuolar sorting signals, primary structure- or physical structure-types exist in soybean seed storage proteins. In the study, we analyzed the sorting mechanism of soybean seed storage protein. We developed transgenic soybean lines accumulating A3B4 subunit alone as 11S globulin, elucidating that physical structure-type sorting signal works in soybean seeds. On the other hand, we prepared crystals of soybean vacuolar sorting receptor with the primary structure-type sorting signal and collected their X-ray diffraction data.
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