| Project/Area Number |
24658290
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子増幅 / 組換え蛋白質生産 / 染色体外遺伝因子 / 細胞がん化 / 細胞工学 / 哺乳動物複製開始 / 複製開始配列 / 核マトリックス結合領域 / IR/MAR / 蛋白質生産 / ゲノム不安定性 / 複製開始 |
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| Research Abstract |
We examined how the IR/MAR sequence, which promote replication initiation, accelerate the gene amplification that was stimulated by the Mtx treatment. Consequently, we uncovered that the IR/MAR sequence enable the initial extrachromosomal maintenance and multiplication of the introduced sequence, which allowed its integration to the multiple chromosomal loci that might be proximity to the chromosomal fragile sites. Thus, the target sequence might efficiently be amplified at the chromosomal context by the BFB cycle, after the Mtx treatments. Our study uncovered the gene amplification mechanism during cancer development. Furthermore, it provided the mechanistic base for the efficient production of protein pharmaceuticals.
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