| Project/Area Number |
24658291
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
ONO Kazuhisa 広島大学, 先端物質科学研究科, 名誉教授 (10144883)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMOTO Seiji 広島大学, 大学院先端物質科学研究科, 准教授 (90294537)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | アレルギー / ミモトープ抗体誘導体 / 免疫療法 / IgEミモトープ / ダニアレルゲン |
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| Research Abstract |
House dust mite allergy is a serious social problem, and establishment of its curable therapy is an urgent issue also in our country. Objective of this study is to develop basic technologies for the generation of sensitized allergen-specific IgE mimotope-human IgG antibody constant region (Fcgamma) chimeric molecules for patient-tailored specific immunotherapy against dust mite allergy. We first developed a new and highly-reproducible method for identification of the IgE mimotope peptides by introducing a pre-screening procedure using allergen-specific IgG from mite-allergic patients. We also developed basic technical strategies for production of recombinant IgE mimotope-Fcgamma chimeras using animal cell expression systems.
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