Development of IgE mimotope-antibody fusion derivatives specific for sensitized allergens

• Project/Area Number: 24658291
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Hiroshima University
• Principal Investigator: ONO Kazuhisa 広島大学, 先端物質科学研究科, 名誉教授 (10144883)
• Co-Investigator(Kenkyū-buntansha): KAWAMOTO Seiji 広島大学, 大学院先端物質科学研究科, 准教授 (90294537)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: アレルギー / ミモトープ抗体誘導体 / 免疫療法 / IgEミモトープ / ダニアレルゲン

Research Abstract

House dust mite allergy is a serious social problem, and establishment of its curable therapy is an urgent issue also in our country. Objective of this study is to develop basic technologies for the generation of sensitized allergen-specific IgE mimotope-human IgG antibody constant region (Fcgamma) chimeric molecules for patient-tailored specific immunotherapy against dust mite allergy. We first developed a new and highly-reproducible method for identification of the IgE mimotope peptides by introducing a pre-screening procedure using allergen-specific IgG from mite-allergic patients. We also developed basic technical strategies for production of recombinant IgE mimotope-Fcgamma chimeras using animal cell expression systems.

Research Products

• [Presentation] Development of Molecular diagnostic system with cedar pollen IgE mimotope (2013)
  • Author(s): Majima M., Okuyama S., Aki T., Kawamoto S., Asaoku Y., Hayashi T., Ono K.
  • Organizer: EAACI-WAO Congress 2013
  • Place of Presentation: ミラノ,イタリア
• [Presentation] Component-resolved molecular diagnosis of house dust mite allergy using a comprehensive repertoire of recombinant mite allergens (2013)
  • Author(s): Kishikawa Y., Kawamoto S., Nakahara H., Watakabe Y., Ota Y., Aki T., Asaoku Y., Hayashi T., Tanaka A., Ono K
  • Organizer: EAACI-WAO Congress 2013
  • Place of Presentation: ミラノ,イタリア
• [Presentation] Development of molecular diagnostic system with cedar pollen IgE mimotopes (2013)
  • Author(s): Masashi Majima
  • Organizer: EAACI-WAO Congress 2013
  • Place of Presentation: イタリア（ミラノ市・ミラノコンベンションセンター）
• [Presentation] ダニアレルギーのcomponent-resolved diagnosis実現に向けた基盤技術の開発(第2報) (2012)
  • Author(s): 中原輝、河本正次、渡壁徳治、岸川由季、秋庸裕、麻奥良子、林鷹治、田中明彦、小埜和久
  • Organizer: 第62回日本アレルギー学会秋季学術大会
  • Place of Presentation: 大阪市