Molecular design, synthesis, and molecular mechanism of MALDI-MS matrices
| Project/Area Number |
24659007
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
SHINDO MITSURU 九州大学, 先導物質化学研究所, 教授 (40226345)
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| Co-Investigator(Renkei-kenkyūsha) |
WARIISHI Hiroyuki 九州大学, 基幹教育院, 教授 (50253513)
MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
FUJIMURA Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 質量分析 / MALDI / マトリックス / 有機合成 / 負イオンモード / アミノ酸 / MALDI法 / 高感度分析 |
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| Research Abstract |
Recently, MALDI-MS has been used for low-molecular-weight metabolite analysis because it is a highly sensitive, high-throughput, and low sample-consuming technique. Although metabolites are predominantly organic acids, negative ionization mode has rarely been utilized due to poor sensitivity with commonly used matrices such as 9-aminoacridine (9-AA). Our purpose is synthesis and evaluation of new matrices for low-molecular metabolite MALDIMS analysis in negative mode. More than 60 compounds including acridine, anthracene and quinoline derivatives were synthesized as MALDI-MS matrix candidates. Using these synthetic matrices, 230 kinds of low-molecular weight metabolites were measured by MALDI-TOF-MS in negative ionization mode. Several new synthetic matrices were found to be more efficient than 9-AA. Especially, aminoanthracene derivatives provided much better analyte signals allowing 51 kinds of amino acid derivatives to be detected in negative mode.
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Report
(3 results)
Research Products
(77 results)
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[Journal Article] Substituent effects of cis-cinnamic acid analogues as plant growh inhibitors2013
Author(s)
Keisuke Nishikawa, Hiroshi Fukuda, Masato Abe, Kazunari Nakanishi, Tomoya Taniguchi, Takashi Nomura, Chihiro Yamaguchi, Syuntaro Hiradate, Yoshiharu Fujii, Katsuhiro Okuda, Mitsuru Shindo
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Journal Title
Phytochemistry
Volume: 96
Pages: 132-147
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular design, synthesis, and evaluation of novel potent apoptosis inhibitors inspired from bongkrekic acid2012
Author(s)
Okuda, K., Hasui, K., Abe, M., Matsumoto, K., Shindo, M
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Journal Title
Chem. Res. Toxicol
Volume: 25
Issue: 10
Pages: 2253-2260
DOI
Related Report
Peer Reviewed
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[Journal Article] Key Structural Features of cis-Cinnamic Acid as an Allelochemical2012
Author(s)
Masato Abe, Keisuke Nishikawa, Hiroshi Fukuda, Kazunari Nakanishi, Yuta Tazawa, Tomoya Taniguchi, So-young Park, Syuntaro Hiradate, Yoshiharu Fujii, Katsuhiro Okuda, Mitsuru Shindo
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Journal Title
Phytochemistry
Volume: 84
Pages: 56-67
DOI
Related Report
Peer Reviewed
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