| Project/Area Number |
24659007
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
SHINDO MITSURU 九州大学, 先導物質化学研究所, 教授 (40226345)
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| Co-Investigator(Renkei-kenkyūsha) |
WARIISHI Hiroyuki 九州大学, 基幹教育院, 教授 (50253513)
MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
FUJIMURA Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 質量分析 / MALDI / マトリックス / 有機合成 / 負イオンモード / アミノ酸 / MALDI法 / 高感度分析 |
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| Research Abstract |
Recently, MALDI-MS has been used for low-molecular-weight metabolite analysis because it is a highly sensitive, high-throughput, and low sample-consuming technique. Although metabolites are predominantly organic acids, negative ionization mode has rarely been utilized due to poor sensitivity with commonly used matrices such as 9-aminoacridine (9-AA). Our purpose is synthesis and evaluation of new matrices for low-molecular metabolite MALDIMS analysis in negative mode. More than 60 compounds including acridine, anthracene and quinoline derivatives were synthesized as MALDI-MS matrix candidates. Using these synthetic matrices, 230 kinds of low-molecular weight metabolites were measured by MALDI-TOF-MS in negative ionization mode. Several new synthetic matrices were found to be more efficient than 9-AA. Especially, aminoanthracene derivatives provided much better analyte signals allowing 51 kinds of amino acid derivatives to be detected in negative mode.
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