| Project/Area Number |
24659008
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
SASAKI Shigeki 九州大学, 薬学研究科(研究院), 教授 (10170672)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 8-ニトログアノシン / 酸化損傷塩基 / 8-オキソグアノシン / 8-ﾆﾄﾛｸﾞｱﾉｼﾝﾓﾉﾘﾝﾘﾝ酸 / 検出 / ８-オキソグアノシン / 8-ニトログアノシンモノリンリン酸 |
|---|
| Research Abstract |
In this study, we designed new thiol-containing recognition molecules for the covalent capture of 8-nitroguanosine based on the Gclamp skeleton by introducing thioalkylurea linker units of varying length (nitroG-grasp). The nitroG-grasp compound with C3 linker exhibited the most efficient guanylation even at low concentrations, supporting the selective complex formation for efficient reactivity for 8-nitro-G. The cyclen unit was attached to G-clamp molecule to recognize 8-oxoGTP, as a model compound of the cyclen-nitroG-grasp molecule. The Zn-complex of the cyclen-oxoG-clamp was able to discriminate 8-oxoGTP from GTP in aqueous media. The cyclen unit was also introduced to the nitroG-grasp molecule, which exhibited guanylation reaction for 8-nitroGMP without forming complex with zinc cation. In conclusion, this study has established the molecular basis for covalent capture of 8-nitroGTP and 8-nitroGMP.
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