Biological roles of fatty acid composition of phospholipids.

• Project/Area Number: 24659025
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Tohoku University
• Principal Investigator: AOKI Junken 東北大学, 薬学研究科(研究院), 教授 (20250219)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: PLA1 / fatty acid / massspectrometry / remodeling / リゾリン脂質 / 脂肪酸 / 多様性 / ホスホリパーゼ / アシルトランスフェラーゼ / GPCR / 生体膜 / 脂肪酸組成 / TGF

Research Abstract

In this study, we examined the mechanism of fatty acid remodeling and its biological roles. We focused two phospholipases called iPLA1a and iPLA1g. We knockdowned the two iPLA1s by RNAi technique in HeLa cells and examined the fatty acid composition of the cells. We found that fatty acid remodening of phospholipid at the sn-1 position was dramatically delayed in iPLA1a-knockdowned HeLa cells. In addition, abnormal mitochondria were frequently observed in the iPLA1a-knockdowned cells. These results indicate that fatty acid remodeling at the sn-1 position of phospholipids by iPLA1a has a critical role in the function of mitochondria.

Research Products

• [Journal Article] TGFα shedding assay: an accurate and versatile method for detecting GPCR activation. (2012)
  • Author(s): Asuka Inoue
  • Journal Title: Nat Methods Volume: 9 Issue: 10 Pages: 1021-1029
  • DOI: 10.1038/nmeth.2172
  • Peer Reviewed
• [Journal Article] The β9 Loop Domain of PA-PLA(1)α Has a Crucial Role in Autosomal Recessive Woolly Hair/Hypotrichosis (2012)
  • Author(s): Shinkuma S, Inoue A, Aoki J, Nishie W, Natsuga K, Ujiie H, Nomura T, Abe R, Akiyama M, Shimizu H
  • Journal Title: The Journal of Investigative Dermatology Volume: (印刷中) Issue: 8 Pages: 2093-2095
  • DOI: 10.1038/jid.2012.96
  • Peer Reviewed
• [Journal Article] The sphingosine-1-phosphate transporter Spns2 expressed on endothelial cells regulates lymphocyte trafficking in mice (2012)
  • Author(s): Fukuhara S, et al
  • Journal Title: J Clin Invest Volume: (In press)(未定) Issue: 4 Pages: 1416-1426
  • DOI: 10.1172/jci60746
  • Peer Reviewed
• [Journal Article] GPR34 is a receptor for lysophosphatidylserine with a fatty acid at the sn-2 position (2012)
  • Author(s): Kitamura H, Makide K, Shuto A, Ikubo M, Inoue A, Suzuki K, Sato Y, Nakamura S, Otani Y, Ohwada T, Aoki J
  • Journal Title: J Biochem Volume: 151 Issue: 5 Pages: 511-518
  • DOI: 10.1093/jb/mvs011
  • NAID: 10031166091
  • Peer Reviewed
• [Presentation] 細胞内型ホスホリパーゼA1の機能解析, Role of intracellular phospholipase A1s in lipid metabolism (2013)
  • Author(s): 中永景太、井上飛鳥、青木淳賢
  • Organizer: 日本生化学会
  • Place of Presentation: 横浜
• [Presentation] 細胞内型ホスホリパーゼA1の機能解析 (2013)
  • Author(s): 中永 景太、井上 飛鳥、青木 淳賢
  • Organizer: 日本生化学会
  • Place of Presentation: 横浜
  • Invited
• [Book] オレオサイエンス (2013)
  • Author(s): 中永景太、井上飛鳥、青木淳賢
  • Total Pages: 613
  • Publisher: 日本油化学会
• [Book] 「ホスホリパーゼA1の構造と機能」オレオサイエンス
  • Author(s): 中永景太、井上飛鳥、青木淳賢
  • Publisher: (Vol. 13)