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A novel method to identify nuclear proteins modified with N-acetylglucosamine by using a soluble glycosyltransferase having nuclear-localization signal

Research Project

Project/Area Number 24659026
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

YAMAMOTO Kazuo  東京大学, 新領域創成科学研究科, 教授 (20174782)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords生化学 / 糖鎖 / 翻訳後修飾 / 核内タンパク質
Research Abstract

Glycosylation of proteins occurs in the lumens of the endoplasmic reticulum and the Golgi apparatus, which resulted in diverse structures of carbohydrate moieties. By contrast, addition of N-acetylglucosamine (GlcNAc) to serine and threonine is a unique glycosylation reaction occurred in nucleus and cytoplasm. We expressed a soluble glycosyltransferase having nuclear-localization signal in the cell and induced further elongation of GlcNAc residue. By using lectin affinity chromatography and mass spectrometry, we comprehensively identified O-GlcNAc modified proteins and their GlcNAc-modified amino acid residues.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (3 results)

All 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Remarks (1 results)

  • [Journal Article] Intracellular lectins are involved in quality control of glycoproteins2014

    • Author(s)
      Yamamoto, K.
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 90 Issue: 2 Pages: 67-82

    • DOI

      10.2183/pjab.90.67

    • NAID

      130003392354

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Terminal N-acetylgalactosamine-specific leguminous lectin from Wisteria japonica as a probe for human lung squamous cell carcinoma.2013

    • Author(s)
      Soga et al.
    • Journal Title

      PLoS ONE

      Volume: 8(12) Issue: 12 Pages: e83886-e83886

    • DOI

      10.1371/journal.pone.0083886

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/iyaku/index.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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