Identification and functional analysis of gamma-secretase-modulating factors
| Project/Area Number |
24659034
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Nagasaki University |
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Principal Investigator |
IWATA Nobuhisa 長崎大学, 医歯薬学総合研究科(薬学系), 教授 (70246213)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | アルツハイマー病 / アミロイドβペプチド / γセクレターゼ / プレセニリン / 活性調節 / γ-セクレターゼ / 亜鉛トランスポーター / 亜鉛 / プロテオリシス |
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| Outline of Final Research Achievements |
We previously found that a production ratio of Aβ42/Aβ40 was decreases in a differentiation day-dependent manner when we differentiated human iPS cells to neuronal cells, suggesting that a γ-secretase-modulating factor may be expressed in the cells, because gene expression of any component of the γ-secretase complex was not changed. So, we analyzed a neuronal differentiation day-dependent gene expression profile and selected some genes as a tentative candidate gene of γ-secretase-modulating factor. We cloned the tentative candidate genes, transfected them into neuronal cells, and measured a production ratio of Aβ42/Aβ40 in cultured medium. To date, we identified one of zinc transporter as γ-secretase-modulating factor. Now, we are planning to confirm this function of the zinc transporter using some different experimental paradigms.
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Report
(4 results)
Research Products
(7 results)
