| Project/Area Number |
24659042
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HANAOKA Kenjiro 東京大学, 薬学研究科(研究院), 准教授 (70451854)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 薬学 / 分析科学 / 有機化学 / in vivo イメージング / 蛍光 / 近赤外蛍光 / イメージング |
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| Outline of Final Research Achievements |
Near-infrared (NIR) fluorescence probes based on the Forster resonance energy transfer (FRET) mechanism have various practical advantages, and their molecular design is generally based on the use of NIR dark quenchers, which are non-fluorescent dyes, as cleavable FRET acceptors. However, few NIR dark quenchers can quench fluorescence in the Cy7 region (over 780 nm). In this research, we developed Si-rhodamine-based NIR dark quenchers (SiNQs), which show broad absorption covering this region. They are non-fluorescent independently of solvent polarity and pH. To demonstrate the usefulness of SiNQs, we designed and synthesized a NIR fluorescence probe for matrix metalloproteinase (MMP) activity using SiNQ780. This probe could detect MMP activity in vitro, in cultured cells and in a tumor-bearing mouse by NIR fluorescence. We believe SiNQs will be useful for the development of a wide range of practical NIR fluorescence probes.
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