| Project/Area Number |
24659044
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
YOKOTA Takanori 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (90231688)
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|---|
| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 医薬分子設計 / アンチセンス / 核酸医薬 / デリバリー / 後根神経節細胞 / ELISA / RT-PCR / PNA / ホスホロチオエート結合修飾 |
|---|
| Research Abstract |
Here we developed a new class of exceptionally potent antisense oligonucleotides (ASOs) that are hybridized with complementary RNA (cRNA) strand and peptide-conjugated PNA strand. At first, we optimized the chemical modifications and length of the strands of the triple-stranded ASO (tsASO). Secondly, peptide-conjugated tsASO reduced mouse TRPV1 gene in dorsal root ganglia (DRG). Finally, we showed the delivery of DRG-delivered peptide-conjugated tsASO using confocal imaging and quantitative RT-PCR by systemic administration.
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