Development of fluorescent probes for metallo-beta-lactamase-producing Gram-negative bacteria
| Project/Area Number |
24659054
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Yoshihiro 熊本大学, 環境安全センター, 准教授 (10363524)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 感染症 / 抗生物質 / 院内感染 / 酵素 / ラクタマーゼ / βーラクタム剤 / 蛍光検出 / 細菌検査 / 細菌感染症 / β‐ラクタム剤 / 金属酵素 |
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| Research Abstract |
Metallo-beta-lactamase can hydrolyze nearly almost beta-lactams, including carbapenems. It is very important to confirm the production of metallo-beta-lactamase in infectious bacteria for effective chemotherapy at the initial stages of a disease. A new fluorogenic probe based on modified cephalosporin was synthesized and examined the ability of the fluorogenic probe to detect metallo-beta-lactamase (IMP-1) by measurement of the fluorescence spectra. As the concentration of IMP-1, the fluorescence intensity of the fluorogenic probe increased. Thus, the spectral data supports the validity of our design strategy. This fluorogenic probe will serve as lead compounds for the development of detection agents that are selective metallo-beta-lactamases-producing infectious bacteria.
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Report
(3 results)
Research Products
(4 results)
